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验证数据展示

  • FC experiment of HepG2 using CL594-67427

    FC experiment of HepG2 using CL594-67427

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human KLK11 (CL594-67427, Clone:1E2B4) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

CoraLite®594-conjugated KLK11 Monoclonal antibody

KLK11 Monoclonal Antibody for FC (Intra)
Cat No. CL594-67427

产品说明书

CloneNo. 1E2B4

宿主/亚型

Mouse / IgG1

种属反应性

Human, Pig, Rat

应用

FC (Intra)

别名

Kallikrein 11, KLK11, PRSS20, Trypsin-like protease, UNQ649/PRO1279, Kallikrein11, Kallikrein-11, Kallikrein-11 inactive chain 1, Kallikrein-11 inactive chain 2, KLK 11, 1E2B4, EC:3.4.21.-

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格价格库存

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经过测试的应用

Positive FC (Intra) detected inHepG2 cells
Positive FC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy KLK11 Ready-To-Use IHC Kit. KLK11 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67427-1-Ig

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-67427 targets KLK11 in FC (Intra) applications and shows reactivity with Human, Pig, Rat samples.

经测试应用 FC (Intra) Application Description
经测试反应性 Human, Pig, Rat
免疫原

CatNo: Ag29895

Product name: Recombinant human KLK11 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 58-250 aa of BC022068

Sequence: LTAAHCLKPRYIVHLGQHNLQKEEGCEQTRTATESFPHPGFNNSLPNKDHRNDIMLVKMASPVSITWAVRPLTLSSRCVTAGTSCLISGWGSTSSPQLRLPHTLRCANITIIEHQKCENAYPGNITDTMVCASVQEGGKDSCQGDSGGPLVCNQSLQGIISWGQDPCAITRKPGVYTKVCKYVDWIQETMKNN

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 kallikrein-related peptidase 11
别名 Kallikrein 11, KLK11, PRSS20, Trypsin-like protease, UNQ649/PRO1279, Kallikrein11, Kallikrein-11, Kallikrein-11 inactive chain 1, Kallikrein-11 inactive chain 2, KLK 11, 1E2B4, EC:3.4.21.-
计算分子量 250 aa, 27 kDa
观测分子量 30-40 kDa
GenBank蛋白编号BC022068
基因名称 KLK11
Gene ID (NCBI) 11012
RRIDAB_3084765
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ9UBX7
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

KLK11(Kallikrein-11) is also named as PRSS20, TLSP and belongs to the peptidase S1 family and Kallikrein subfamily. It is a multifunctinal protease cleaving several substrates for kallikrein and trypsin and involved in normal physiology processes in bronchus. This full length protein has a signal peptide, a propeptide and four glycosylation sites. It has 4 isoforms produced by alternative splicing with the molecular weight of 31 kDa, 27 kDa,34 kDa and 30 kDa.

实验方案

Product Specific Protocols
FC protocol for CL594 KLK11 antibody CL594-67427Download protocol
Standard Protocols
Click here to view our Standard Protocols