KLK11 Monoclonal antibody
KLK11 Monoclonal Antibody for IF-P, IHC, WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human, Pig, Rat and More (1)
Applications
IF-P, IHC, WB, ELISA
Conjugate
Unconjugated
CloneNo.
1E2B4
验证数据展示
经过测试的应用
| Positive WB detected in | DU 145 cells, pig brain tissue, LNCaP cells, PC-3 cells, Jurkat cells, rat brain tissue |
| Positive IHC detected in | human breast cancer tissue, human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | human breast cancer tissue |
Planning an IHC experiment? We recommend our IHCeasy KLK11 Ready-To-Use IHC Kit. KLK11 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF) | IF : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
| IHC | See 1 publications below |
产品信息
67427-1-Ig targets KLK11 in WB, IF, IHC, ELISA applications and shows reactivity with Human, Pig, Rat samples.
| Tested Applications | IF-P, IHC, WB, ELISA |
| Cited Applications | WB, IHC |
| Tested Reactivity | Human, Pig, Rat |
| Cited Reactivity | human |
| Immunogen | KLK11 fusion protein Ag29895 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | kallikrein-related peptidase 11 |
| Synonyms | Hippostasin, hK11, Kallikrein 11, KLK11, PRSS20, Serine protease 20, TLSP, Trypsin like protease |
| Calculated Molecular Weight | 250 aa, 27 kDa |
| Observed Molecular Weight | 30-40 kDa |
| GenBank Accession Number | BC022068 |
| Gene Symbol | KLK11 |
| Gene ID (NCBI) | 11012 |
| RRID | AB_2882666 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q9UBX7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Aliquoting is unnecessary for -20oC storage. |
背景介绍
KLK11(Kallikrein-11) is also named as PRSS20, TLSP and belongs to the peptidase S1 family and Kallikrein subfamily. It is a multifunctinal protease cleaving several substrates for kallikrein and trypsin and involved in normal physiology processes in bronchus. This full length protein has a signal peptide, a propeptide and four glycosylation sites. It has 4 isoforms produced by alternative splicing with the molecular weight of 31 kDa, 27 kDa,34 kDa and 30 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for KLK11 antibody 67427-1-Ig | Download protocol |
| IHC protocol for KLK11 antibody 67427-1-Ig | Download protocol |
| IF protocol for KLK11 antibody 67427-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Bioenerg Biomembr KLK11 acts as a tumor-inhibitor in laryngeal squamous cell carcinoma through the inactivation of Akt/Wnt/β-catenin signaling. | ||
Carcinogenesis miR-1304 targets KLK11 to regulate gastric cancer cell proliferation through the mTOR signaling pathway |
