验证数据展示
经过测试的应用
| Positive WB detected in | TT cells, DU 145 cells, pig brain tissue, LNCaP cells, PC-3 cells, Jurkat cells, rat brain tissue, A431 cells, HaCaT cells, HT-29 cells, SW 1990 cells, mouse brain tissue, rat skin tissue |
| Positive IHC detected in | human breast cancer tissue, human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human breast cancer tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
| IHC | See 1 publications below |
产品信息
67427-1-Ig targets KLK11 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
| 经测试应用 | WB, IHC, IF-P, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse, rat, pig |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag29895 Product name: Recombinant human KLK11 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 58-250 aa of BC022068 Sequence: LTAAHCLKPRYIVHLGQHNLQKEEGCEQTRTATESFPHPGFNNSLPNKDHRNDIMLVKMASPVSITWAVRPLTLSSRCVTAGTSCLISGWGSTSSPQLRLPHTLRCANITIIEHQKCENAYPGNITDTMVCASVQEGGKDSCQGDSGGPLVCNQSLQGIISWGQDPCAITRKPGVYTKVCKYVDWIQETMKNN 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | kallikrein-related peptidase 11 |
| 别名 | Kallikrein 11, 1E2B4, EC:3.4.21.-, Kallikrein11, Kallikrein-11 |
| 计算分子量 | 250 aa, 27 kDa |
| 观测分子量 | 30-40 kDa |
| GenBank蛋白编号 | BC022068 |
| 基因名称 | KLK11 |
| Gene ID (NCBI) | 11012 |
| RRID | AB_2882666 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | Q9UBX7 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
KLK11(Kallikrein-11) is also named as PRSS20, TLSP and belongs to the peptidase S1 family and Kallikrein subfamily. It is a multifunctinal protease cleaving several substrates for kallikrein and trypsin and involved in normal physiology processes in bronchus. This full length protein has a signal peptide, a propeptide and four glycosylation sites. It has 4 isoforms produced by alternative splicing with the molecular weight of 31 kDa, 27 kDa,34 kDa and 30 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for KLK11 antibody 67427-1-Ig | Download protocol |
| IHC protocol for KLK11 antibody 67427-1-Ig | Download protocol |
| WB protocol for KLK11 antibody 67427-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Bioenerg Biomembr KLK11 acts as a tumor-inhibitor in laryngeal squamous cell carcinoma through the inactivation of Akt/Wnt/β-catenin signaling. | ||
Carcinogenesis miR-1304 targets KLK11 to regulate gastric cancer cell proliferation through the mTOR signaling pathway |
