验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293T cells, A431 cells, MCF-7 cells, K-562 cells |
| Positive IF/ICC detected in | MCF-7 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
85893-1-RR targets KLHL2 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag5950 Product name: Recombinant human KLHL2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 273-593 aa of BC022503 Sequence: DYLIEAMKYHLLPTEQRILMKSVRTRLRTPMNLPKLMVVVGGQAPKAIRSVECYDFKEERWHQVAELPSRRCRAGMVYMAGLVFAVGGFNGSLRVRTVDSYDPVKDQWTSVANMRDRRSTLGAAVLNGLLYAVGGFDGSTGLSSVEAYNIKSNEWFHVAPMNTRRSSVGVGVVGGLLYAVGGYDVASRQCLSTVECYNATTNEWTYIAEMSTRRSGAGVGVLNNLLYAVGGHDGPLVRKSVEVYDPTTNAWRQVADMNMCRRNAGVCAVNGLLYVVGGDDGSCNLASVEYYNPTTDKWTVVSSCMSTGRSYAGVTVIDKRL 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | kelch-like 2, Mayven (Drosophila) |
| 别名 | ABP KELCH, Actin-binding protein Mayven, Kelch-like protein 2 |
| 计算分子量 | 66 kDa |
| 观测分子量 | 66 kDa |
| GenBank蛋白编号 | BC022503 |
| 基因名称 | KLHL2 |
| Gene ID (NCBI) | 11275 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | O95198 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
KLHL2 contains an SH3-binding domain in the N terminus and a POZ domain and 6 tandem repeats of approximately 50 amino acids each in the C terminus. In astrocytoma/glioblastoma cells, KLHL2 colocalized with actin filaments in the cytoskeleton remodeling regions in stress fibers and in patchy cortical actin-rich regions of the cell margins. In primary rat hippocampal neurons, it was highly expressed in the cell body and in neurite processes(PMID: 10397770).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for KLHL2 antibody 85893-1-RR | Download protocol |
| WB protocol for KLHL2 antibody 85893-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
