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验证数据展示

  • WB analysis using 85893-1-RR (same clone as 85893-1-PBS)

    WB analysis using 85893-1-RR (same clone as 85893-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85893-1-RR (KLHL2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85893-1-PBS in a different storage buffer formulation.

  • IF Staining of MCF-7 using 85893-1-RR (same clone as 85893-1-PBS)

    IF Staining of MCF-7 using 85893-1-RR (same clone as 85893-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using KLHL2 antibody (85893-1-RR, Clone: 250148G5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 85893-1-PBS in a different storage buffer formulation.

KLHL2 Recombinant monoclonal antibody, PBS Only

KLHL2 Uni-rAb® Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 85893-1-PBS

产品说明书

CloneNo. 250148G5

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, Indirect ELISA

别名

ABP KELCH, Actin-binding protein Mayven, Kelch-like protein 2

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

85893-1-PBS targets KLHL2 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag5950

Product name: Recombinant human KLHL2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 273-593 aa of BC022503

Sequence: DYLIEAMKYHLLPTEQRILMKSVRTRLRTPMNLPKLMVVVGGQAPKAIRSVECYDFKEERWHQVAELPSRRCRAGMVYMAGLVFAVGGFNGSLRVRTVDSYDPVKDQWTSVANMRDRRSTLGAAVLNGLLYAVGGFDGSTGLSSVEAYNIKSNEWFHVAPMNTRRSSVGVGVVGGLLYAVGGYDVASRQCLSTVECYNATTNEWTYIAEMSTRRSGAGVGVLNNLLYAVGGHDGPLVRKSVEVYDPTTNAWRQVADMNMCRRNAGVCAVNGLLYVVGGDDGSCNLASVEYYNPTTDKWTVVSSCMSTGRSYAGVTVIDKRL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 kelch-like 2, Mayven (Drosophila)
别名 ABP KELCH, Actin-binding protein Mayven, Kelch-like protein 2
计算分子量 66 kDa
观测分子量 66 kDa
GenBank蛋白编号BC022503
基因名称 KLHL2
Gene ID (NCBI) 11275
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDO95198
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

KLHL2 contains an SH3-binding domain in the N terminus and a POZ domain and 6 tandem repeats of approximately 50 amino acids each in the C terminus. In astrocytoma/glioblastoma cells, KLHL2 colocalized with actin filaments in the cytoskeleton remodeling regions in stress fibers and in patchy cortical actin-rich regions of the cell margins. In primary rat hippocampal neurons, it was highly expressed in the cell body and in neurite processes(PMID: 10397770).

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