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CoraLite®594-conjugated IL-1R2 Monoclonal antibody

IL-1R2 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse

Applications

IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

3H4H4

Cat No : CL594-60262

Print datasheet

Synonyms

CD121b, CDw121b, IL 1R 2, IL 1R beta, IL 1RT 2, IL 1RT2, IL1R2, IL1RB, Interleukin 1 receptor beta, Interleukin 1 receptor type 2, Interleukin 1 receptor type II

验证数据展示

  • IF Staining of HeLa using CL594-60262

    IF Staining of HeLa using CL594-60262

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite®594-conjugated IL1R2 antibody (CL594-60262, Clone: 3H4H4 ) at dilution of 1:100.

经过测试的应用

Positive IF detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 60262-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of CL594-60262 is IL-1R2 Fusion Protein expressed in E. coli.

Tested Applications IF/ICC
Tested Reactivity human, mouse
Immunogen IL-1R2 fusion protein Ag4495 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name interleukin 1 receptor, type II
Synonyms CD121b, CDw121b, IL 1R 2, IL 1R beta, IL 1RT 2, IL 1RT2, IL1R2, IL1RB, Interleukin 1 receptor beta, Interleukin 1 receptor type 2, Interleukin 1 receptor type II
Calculated Molecular Weight 398 aa, 45 kDa
Observed Molecular Weight 45 kDa
GenBank Accession NumberBC039031
Gene Symbol IL1R2
Gene ID (NCBI) 7850
RRIDAB_2883451
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP27930
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Interleukin-1 receptor type 2 (IL1R2, also known as CD121b) is a cytokine receptor that belongs to the interleukin 1 receptor family. It exists in both a membrane bound and soluble form. IL1R2 binds interleukin alpha (IL1A), interleukin beta (IL1B), and interleukin 1 receptor, type I(IL1R1/IL1RA), and acts as a decoy receptor that inhibits the activity of its ligands. IL1R2 serves as a negative regulator of IL-1 signaling, thereby suppressing inflammatory response.

实验方案

Product Specific Protocols
IF protocol for CL594 IL-1R2 antibody CL594-60262Download protocol
Standard Protocols
Click here to view our Standard Protocols