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验证数据展示

  • IF Staining of HeLa using CL594-60262

    IF Staining of HeLa using CL594-60262

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite®594-conjugated IL1R2 antibody (CL594-60262, Clone: 3H4H4 ) at dilution of 1:100.

CoraLite®594-conjugated IL-1R2 Monoclonal antibody

IL-1R2 Monoclonal Antibody for IF/ICC
Cat No. CL594-60262

产品说明书

CloneNo. 3H4H4

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse

应用

IF/ICC

别名

CD121b, CDw121b, IL 1R 2, IL 1R beta, IL 1RT 2, IL 1RT2, IL1R2, IL1RB, Interleukin 1 receptor beta, Interleukin 1 receptor type 2, Interleukin 1 receptor type II

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 60262-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-60262 targets IL-1R2 in IF/ICC applications and shows reactivity with human, mouse samples.

经测试应用 IF/ICC Application Description
经测试反应性 human, mouse
免疫原 IL-1R2 fusion protein Ag4495 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 interleukin 1 receptor, type II
别名 CD121b, CDw121b, IL 1R 2, IL 1R beta, IL 1RT 2, IL 1RT2, IL1R2, IL1RB, Interleukin 1 receptor beta, Interleukin 1 receptor type 2, Interleukin 1 receptor type II
计算分子量 398 aa, 45 kDa
观测分子量 45 kDa
GenBank蛋白编号BC039031
基因名称 IL1R2
Gene ID (NCBI) 7850
RRIDAB_2883451
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP27930
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Interleukin-1 receptor type 2 (IL1R2, also known as CD121b) is a cytokine receptor that belongs to the interleukin 1 receptor family. It exists in both a membrane bound and soluble form. IL1R2 binds interleukin alpha (IL1A), interleukin beta (IL1B), and interleukin 1 receptor, type I(IL1R1/IL1RA), and acts as a decoy receptor that inhibits the activity of its ligands. IL1R2 serves as a negative regulator of IL-1 signaling, thereby suppressing inflammatory response.

实验方案

Product Specific Protocols
IF protocol for CL594 IL-1R2 antibody CL594-60262Download protocol
Standard Protocols
Click here to view our Standard Protocols