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验证数据展示

  • WB analysis using 20253-1-AP (same clone as 20253-1-PBS)

    WB analysis using 20253-1-AP (same clone as 20253-1-PBS)

    HepG2 cells lysates were subjected to SDS PAGE followed by western blot with 20253-1-AP (IGF2R-Specific antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20253-1-PBS in a different storage buffer formulation.

  • WB analysis of HepG2 using 20253-1-AP (same clone as 20253-1-PBS)

    WB analysis of HepG2 using 20253-1-AP (same clone as 20253-1-PBS)

    WB result of IGF2R-Specific antibody (20253-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-IGF2R-Specific transfected HepG2 cells. This data was developed using the same antibody clone with 20253-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse colon using 20253-1-AP (same clone as 20253-1-PBS)

    IHC staining of mouse colon using 20253-1-AP (same clone as 20253-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 20253-1-AP (IGF2R-Specific antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20253-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with IGF2R antibody included
  • IHC staining of mouse colon using 20253-1-AP (same clone as 20253-1-PBS)

    IHC staining of mouse colon using 20253-1-AP (same clone as 20253-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 20253-1-AP (IGF2R-Specific antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20253-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with IGF2R antibody included
  • FC experiment of HepG2 using 20253-1-AP (same clone as 20253-1-PBS)

    FC experiment of HepG2 using 20253-1-AP (same clone as 20253-1-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug IGF2R-Specific Polyclonal antibody (20253-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 20253-1-PBS in a different storage buffer formulation.

  • IP experiment of HepG2 using 20253-1-AP (same clone as 20253-1-PBS)

    IP experiment of HepG2 using 20253-1-AP (same clone as 20253-1-PBS)

    IP result of anti-IGF2R-Specific (IP:20253-1-AP, 4ug; Detection:20253-1-AP 1:500) with HepG2 cells lysate 3480ug. This data was developed using the same antibody clone with 20253-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 20253-1-AP (same clone as 20253-1-PBS)

    IF Staining of HepG2 using 20253-1-AP (same clone as 20253-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using IGF2R-Specific antibody (20253-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 20253-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

IGF2R-Specific Polyclonal antibody, PBS Only

IGF2R-Specific Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA
Cat No. 20253-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

别名

IGF2R, CI MPR, CI Man-6-P receptor, CI Man 6 P receptor, CD222

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy IGF2R Ready-To-Use IHC Kit. IGF2R primary antibody included.

产品信息

20253-1-PBS targets IGF2R-Specific in WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 insulin-like growth factor 2 receptor
别名 IGF2R, CI MPR, CI Man-6-P receptor, CI Man 6 P receptor, CD222
计算分子量 274 kDa
观测分子量 300 kDa
GenBank蛋白编号NM_000876
基因名称 IGF2R
Gene ID (NCBI) 3482
RRIDAB_10859779
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP11717
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

IGF2R, also named as MPRI, CI Man-6-P receptor, CI-MPR, M6PR, IGF-II receptor, M6P/IGF2R, MPR 300 and CD222, belongs to the MRL1/IGF2R family. IGF2R transports of phosphorylated lysosomal enzymes from the Golgi complex and the cell surface to lysosomes. IGF2R is a lysosomal enzymes bearing phosphomannosyl residues bind specifically to mannose-6-phosphate receptors in the Golgi apparatus and the resulting receptor-ligand complex is transported to an acidic prelyosomal compartment where the low pH mediates the dissociation of the complex. This receptor also binds IGF2. IGF2R acts as a positive regulator of T-cell coactivation, by binding DPP4. The antibody is specific to IGF2R.

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