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IGF2R-Specific Polyclonal antibody

IGF2R-Specific Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Cat No. 20253-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse and More (2)

应用

WB, IHC, IF/ICC, FC (Intra), IP, ELISA and More (1)

IGF2R, CI MPR, CI Man-6-P receptor, CI Man 6 P receptor, CD222

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格价格库存


经过测试的应用

Positive WB detected inHepG2 cells, A431 cells, L02 cells
Positive IP detected inHepG2 cells
Positive IHC detected inmouse colon tissue, human liver tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy IGF2R Ready-To-Use IHC Kit. IGF2R primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite® Plus 647 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20253-1-AP targets IGF2R-Specific in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human, mouse
文献引用反应性human, mouse, rat, bovine
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 insulin-like growth factor 2 receptor
别名 IGF2R, CI MPR, CI Man-6-P receptor, CI Man 6 P receptor, CD222
计算分子量 274 kDa
观测分子量 300 kDa
GenBank蛋白编号NM_000876
基因名称 IGF2R
Gene ID (NCBI) 3482
RRIDAB_10859779
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP11717
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IGF2R, also named as MPRI, CI Man-6-P receptor, CI-MPR, M6PR, IGF-II receptor, M6P/IGF2R, MPR 300 and CD222, belongs to the MRL1/IGF2R family. IGF2R transports of phosphorylated lysosomal enzymes from the Golgi complex and the cell surface to lysosomes. IGF2R is a lysosomal enzymes bearing phosphomannosyl residues bind specifically to mannose-6-phosphate receptors in the Golgi apparatus and the resulting receptor-ligand complex is transported to an acidic prelyosomal compartment where the low pH mediates the dissociation of the complex. This receptor also binds IGF2. IGF2R acts as a positive regulator of T-cell coactivation, by binding DPP4. The antibody is specific to IGF2R.

实验方案

Product Specific Protocols
WB protocol for IGF2R-Specific antibody 20253-1-APDownload protocol
IHC protocol for IGF2R-Specific antibody 20253-1-APDownload protocol
IF protocol for IGF2R-Specific antibody 20253-1-APDownload protocol
IP protocol for IGF2R-Specific antibody 20253-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Hum Mol Genet

Progranulin regulates lysosomal function and biogenesis through acidification of lysosomes.

Authors - Yoshinori Tanaka
ratWB

J Agric Food Chem

Mitotically stable modification of DNA methylation in IGF2/H19 imprinting control region is associated with activated hepatic IGF2 expression in offspring rats from betaine-supplemented dams.

Authors - Shu Yang
humanWB, IF

Mol Biol Cell

Hydrogen peroxide induces Arl1 degradation and impairs Golgi-mediated trafficking.

Authors - Stephen C Ireland
humanWB

J Cancer

Trop2 binding IGF2R induces gefitinib resistance in NSCLC by remodeling the tumor microenvironment.

Authors - Xia Sun
humanWB

Life Sci Alliance

Identification and characterization of a membrane receptor that binds to human STC1.

Authors - Hin Ting Wan
ratWB,IHC

J Sci Food Agric

Grandmaternal betaine supplementation enhances hepatic IGF2 expression in F2 rat offspring through modification of promoter DNA methylation.

Authors - Shu Yang
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