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验证数据展示

  • FC experiment of HepG2 using CL488-20253

    FC experiment of HepG2 using CL488-20253

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human IGF2R-Specific (CL488-20253) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite® Plus 488-conjugated IGF2R-Specific Polyclonal antibody

IGF2R-Specific Polyclonal Antibody for FC (Intra)
Cat No. CL488-20253

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

FC (Intra)

别名

IGF2R, CI MPR, CI Man-6-P receptor, CI Man 6 P receptor, CD222

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy IGF2R Ready-To-Use IHC Kit. IGF2R primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 20253-1-AP

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-20253 targets IGF2R-Specific in FC (Intra) applications and shows reactivity with human samples.

经测试应用 FC (Intra) Application Description
经测试反应性 human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 insulin-like growth factor 2 receptor
别名 IGF2R, CI MPR, CI Man-6-P receptor, CI Man 6 P receptor, CD222
计算分子量 274 kDa
观测分子量 300 kDa
GenBank蛋白编号NM_000876
基因名称 IGF2R
Gene ID (NCBI) 3482
RRIDAB_2919169
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP11717
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IGF2R, also named as MPRI, CI Man-6-P receptor, CI-MPR, M6PR, IGF-II receptor, M6P/IGF2R, MPR 300 and CD222, belongs to the MRL1/IGF2R family. IGF2R transports of phosphorylated lysosomal enzymes from the Golgi complex and the cell surface to lysosomes. IGF2R is a lysosomal enzymes bearing phosphomannosyl residues bind specifically to mannose-6-phosphate receptors in the Golgi apparatus and the resulting receptor-ligand complex is transported to an acidic prelyosomal compartment where the low pH mediates the dissociation of the complex. This receptor also binds IGF2. IGF2R acts as a positive regulator of T-cell coactivation, by binding DPP4. The antibody is specific to IGF2R.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 IGF2R-Specific antibody CL488-20253Download protocol
Standard Protocols
Click here to view our Standard Protocols