验证数据展示
经过测试的应用
| Positive WB detected in | Control |
| Positive IP detected in | Control |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
30000-0-AP targets Rabbit IgG control in WB, IHC, IP, CoIP, ChIP, RIP, IP-MS, ELISA, Blocking assay, Cell treatment applications and shows reactivity with n/a samples.
| 经测试应用 | WB, IP, ELISA Application Description |
| 文献引用应用 | IHC, IP, CoIP, ChIP, RIP, IP-MS, ELISA, Blocking assay, Cell treatment |
| 经测试反应性 | n/a |
| 文献引用反应性 | hamster |
| 免疫原 |
N/A 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | Rabbit IgG control |
| 别名 | IgG, IgG control, Isotype control, Rabbit IgG, Rabbit IgG isotype |
| 基因名称 | |
| Gene ID (NCBI) | |
| RRID | AB_2819035 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A affinity purification |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
This product is Normal rabbit IgG (without immunized) which purified with Protein A. Normal Rabbit IgG is an isotype control antibody, which is used to estimate the non-specific binding of target primary antibodies due to Fc receptor binding or other protein-protein interactions. An isotype control antibody should have the same immunoglobulin type and be used at the same concentration as the test antibody.
发表文章
| Species | Application | Title |
|---|---|---|
Signal Transduct Target Ther Glibenclamide targets MDH2 to relieve aging phenotypes through metabolism-regulated epigenetic modification | ||
Signal Transduct Target Ther Krüppel-like factor 4 downregulation during esophageal cancer formation facilitates immune evasion and impairs immunotherapy | ||
Cancer Cell Endocytic evasion confers resistance to antibody-drug conjugates therapy in cancer. | ||
Cancer Cell Lipid oxidation reprogramming in cancer-associated fibroblasts enhances CD8(+) T cell cytotoxicity and therapeutic response. | ||
Cancer Cell Targeting tumor monocyte-intrinsic PD-L1 by rewiring STING signaling and enhancing STING agonist therapy |
