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CoraLite®594-conjugated Glycophorin A Monoclonal antibody

Glycophorin A Monoclonal Antibody for IF-P

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

IF-P

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

2B12G10

Cat No : CL594-66778

Print datasheet

Synonyms

CD235a, Glycophorin A, GPA, GPErik, GpMiIII, GPSAT, GYPA, HGpMiIII, HGpMiV, HGpMiX, HGpMiXI, HGpSta(C), MN, MN sialoglycoprotein, MNS, PAS 2, Sialoglycoprotein alpha

验证数据展示

  • IF Staining of human placenta using CL594-66778

    IF Staining of human placenta using CL594-66778

    Immunofluorescent analysis of (4% PFA) fixed human placenta tissue using CoraLite®594 Glycophorin A antibody (CL594-66778, Clone: 2B12G10 ) at dilution of 1:200.

  • IF Staining of human placenta using CL594-66778

    IF Staining of human placenta using CL594-66778

    Immunofluorescent analysis of (4% PFA) fixed human placenta tissue using CoraLite®594 Glycophorin A antibody (CL594-66778, Clone: 2B12G10 ) at dilution of 1:200.

经过测试的应用

Positive IF detected inhuman placenta tissue
Planning an IHC experiment? We recommend our IHCeasy Glycophorin A Ready-To-Use IHC Kit. Glycophorin A primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66778-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66778 targets Glycophorin A in IF applications and shows reactivity with Human samples.

Tested Applications IF-P
Tested Reactivity Human
Immunogen Glycophorin A fusion protein Ag8635 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name glycophorin A (MNS blood group)
Synonyms CD235a, Glycophorin A, GPA, GPErik, GpMiIII, GPSAT, GYPA, HGpMiIII, HGpMiV, HGpMiX, HGpMiXI, HGpSta(C), MN, MN sialoglycoprotein, MNS, PAS 2, Sialoglycoprotein alpha
Calculated Molecular Weight 150 aa, 16 kDa
Observed Molecular Weight 36-38 kDa
GenBank Accession NumberBC005319
Gene Symbol GYPA
Gene ID (NCBI) 2993
RRIDAB_2920035
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP02724
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

背景介绍

Glycophorin A (GYPA) is the major transmembrane sialoglycoprotein in erythrocytes. It is a dimeric type I transmembrane protein carrying 15 closely clustered O-linked tetrasaccharides capped with sialic acid/N-acetylneuraminic acid (Neu5Ac). This 36 kDa protein represents the major sialoglycoprotein of the red blood cell membrane displaying about one million copies per cell. (PMID: 9490702)

实验方案

Product Specific Protocols
IF protocol for CL594 Glycophorin A antibody CL594-66778Download protocol
Standard Protocols
Click here to view our Standard Protocols