• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

Glycophorin A Monoclonal antibody

Glycophorin A Monoclonal Antibody for FC, IF-P, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human and More (1)

Applications

FC, IF-P, IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

2B12G10

Cat No : 66778-1-Ig

Print datasheet

Synonyms

CD235a, Glycophorin A, GPA, GPErik, GpMiIII, GPSAT, GYPA, HGpMiIII, HGpMiV, HGpMiX, HGpMiXI, HGpSta(C), MN, MN sialoglycoprotein, MNS, PAS 2, Sialoglycoprotein alpha

验证数据展示

  • WB analysis of human blood using 66778-1-Ig

    WB analysis of human blood using 66778-1-Ig

    human blood were subjected to SDS PAGE followed by western blot with 66778-1-Ig (GYPA antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human placenta using 66778-1-Ig

    IHC staining of human placenta using 66778-1-Ig

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66778-1-Ig (Glycophorin A antibody) at dilution of 1:20000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Glycophorin A antibody included
  • IHC staining of human placenta using 66778-1-Ig

    IHC staining of human placenta using 66778-1-Ig

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66778-1-Ig (Glycophorin A antibody) at dilution of 1:20000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Glycophorin A antibody included
  • IF Staining of human placenta using 66778-1-Ig

    IF Staining of human placenta using 66778-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human placenta tissue using Glycophorin A antibody (66778-1-Ig, Clone: 2B12G10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • IF Staining of human placenta using 66778-1-Ig

    IF Staining of human placenta using 66778-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human placenta tissue using Glycophorin A antibody (66778-1-Ig, Clone: 2B12G10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • FC experiment of K-562 using 66778-1-Ig

    FC experiment of K-562 using 66778-1-Ig

    1X10^6 K-562 cells were intracellularly stained with 0.4 ug Anti-Human Glycophorin A (66778-1-Ig, Clone:2B12G10) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inhuman blood tissue
Positive IHC detected inhuman placenta tissue, human stomach cancer tissue, human spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman placenta tissue
Positive FC detected inK-562 cells
Planning an IHC experiment? We recommend our IHCeasy Glycophorin A Ready-To-Use IHC Kit. Glycophorin A primary antibody included.
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:6000
Immunohistochemistry (IHC)IHC : 1:10000-1:40000
Immunofluorescence (IF)IF : 1:200-1:800
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

66778-1-Ig targets Glycophorin A in WB, IF, FC, IHC, ELISA applications and shows reactivity with Human samples.

Tested Applications FC, IF-P, IHC, WB, ELISA
Cited ApplicationsWB
Tested Reactivity Human
Cited Reactivityhuman
Immunogen Glycophorin A fusion protein Ag8635 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name glycophorin A (MNS blood group)
Synonyms CD235a, Glycophorin A, GPA, GPErik, GpMiIII, GPSAT, GYPA, HGpMiIII, HGpMiV, HGpMiX, HGpMiXI, HGpSta(C), MN, MN sialoglycoprotein, MNS, PAS 2, Sialoglycoprotein alpha
Calculated Molecular Weight 150 aa, 16 kDa
Observed Molecular Weight 36-38 kDa
GenBank Accession NumberBC005319
Gene Symbol GYPA
Gene ID (NCBI) 2993
RRIDAB_2882124
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP02724
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Glycophorin A (GYPA) is the major transmembrane sialoglycoprotein in erythrocytes. It is a dimeric type I transmembrane protein carrying 15 closely clustered O-linked tetrasaccharides capped with sialic acid/N-acetylneuraminic acid (Neu5Ac). This 36 kDa protein represents the major sialoglycoprotein of the red blood cell membrane displaying about one million copies per cell. (PMID: 9490702)

实验方案

Product Specific Protocols
WB protocol for Glycophorin A antibody 66778-1-IgDownload protocol
IHC protocol for Glycophorin A antibody 66778-1-IgDownload protocol
IF protocol for Glycophorin A antibody 66778-1-IgDownload protocol
FC protocol for Glycophorin A antibody 66778-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Biochem

C-terminally truncation is a prominent post-translational modification of human erythrocyte α-synuclein

Authors - Ryosuke Amagai
View Article