验证数据展示
经过测试的应用
Positive IF/ICC detected in | A431 cells |
推荐稀释比
应用 | 推荐稀释比 |
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Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL488-68233 targets GPSM1 in IF/ICC applications and shows reactivity with Human, mouse samples.
经测试应用 | IF/ICC Application Description |
经测试反应性 | Human, mouse |
免疫原 | GPSM1 fusion protein Ag18773 种属同源性预测 |
宿主/亚型 | Mouse / IgG1 |
抗体类别 | Monoclonal |
产品类型 | Antibody |
全称 | G-protein signaling modulator 1 (AGS3-like, C. elegans) |
别名 | AGS3, DKFZp727I051, GPSM1 |
计算分子量 | 652 aa, 72 kDa |
观测分子量 | 75 kDa |
GenBank蛋白编号 | BC017353 |
基因名称 | GPSM1 |
Gene ID (NCBI) | 26086 |
RRID | AB_3084449 |
偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
最大激发/发射波长 | 493 nm / 522 nm |
形式 | Liquid |
纯化方式 | Protein G purification |
UNIPROT ID | Q86YR5 |
储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GPSM1, also named as AGS3, is a receptor-independent G protein activator that has been implicated in multiple biological events such as brain development, neuroplasticity and addiction, cardiac function, Golgi structure/function, macroautophagy and metabolism. It contains seven tetratricopeptide repeats in its N-terminal half and four G-protein regulatory (GPR) motifs in its C-terminal half. It has been shown that AGS3 could regulate the orientation of the mitotic spindle, cAMP production, membrane protein transport, and asymmetric cell division by binding preferentially to inactive Gai/o subunits complexed with guanine dinucleotide phosphate (GDP) at multiple G-protein regulatory or GoLoco motif repeats. It also plays an important anti-apoptotic role through enhancing the phosphorylation of cyclic AMP response element-binding protein (p-CREB).
实验方案
Product Specific Protocols | |
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IF protocol for CL Plus 488 GPSM1 antibody CL488-68233 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |