CoraLite® Plus 488-conjugated GPSM1 Monoclonal antibody

GPSM1 Monoclonal Antibody for IF/ICC
Cat No. CL488-68233

产品说明书

CloneNo. 3A7A2

宿主/亚型

Mouse / IgG1

种属反应性

Human, mouse

应用

IF/ICC

AGS3, DKFZp727I051, GPSM1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -


经过测试的应用

Positive IF/ICC detected inA431 cells
For other applications, we recommend the unconjugated version of this antibody, 68233-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-68233 targets GPSM1 in IF/ICC applications and shows reactivity with Human, mouse samples.

经测试应用 IF/ICC Application Description
经测试反应性 Human, mouse
免疫原 GPSM1 fusion protein Ag18773 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 G-protein signaling modulator 1 (AGS3-like, C. elegans)
别名 AGS3, DKFZp727I051, GPSM1
计算分子量 652 aa, 72 kDa
观测分子量 75 kDa
GenBank蛋白编号BC017353
基因名称 GPSM1
Gene ID (NCBI) 26086
RRIDAB_3084449
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ86YR5
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GPSM1, also named as AGS3, is a receptor-independent G protein activator that has been implicated in multiple biological events such as brain development, neuroplasticity and addiction, cardiac function, Golgi structure/function, macroautophagy and metabolism. It contains seven tetratricopeptide repeats in its N-terminal half and four G-protein regulatory (GPR) motifs in its C-terminal half. It has been shown that AGS3 could regulate the orientation of the mitotic spindle, cAMP production, membrane protein transport, and asymmetric cell division by binding preferentially to inactive Gai/o subunits complexed with guanine dinucleotide phosphate (GDP) at multiple G-protein regulatory or GoLoco motif repeats. It also plays an important anti-apoptotic role through enhancing the phosphorylation of cyclic AMP response element-binding protein (p-CREB).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 GPSM1 antibody CL488-68233Download protocol
Standard Protocols
Click here to view our Standard Protocols
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