GPSM1 Monoclonal antibody

GPSM1 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 68233-1-Ig

产品说明书

CloneNo. 3A7A2

宿主/亚型

Mouse / IgG1

种属反应性

Human, mouse, rat

应用

WB, IF/ICC, ELISA

AGS3, DKFZp727I051, GPSM1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -


经过测试的应用

Positive WB detected inLNCaP cells, HeLa cells, HEK-293 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells
Positive IF/ICC detected inPC-12 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68233-1-Ig targets GPSM1 in WB, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 Human, mouse, rat
免疫原 GPSM1 fusion protein Ag18773 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 G-protein signaling modulator 1 (AGS3-like, C. elegans)
别名 AGS3, DKFZp727I051, GPSM1
计算分子量 652 aa, 72 kDa
观测分子量 75 kDa
GenBank蛋白编号BC017353
基因名称 GPSM1
Gene ID (NCBI) 26086
RRIDAB_2935320
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ86YR5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GPSM1, also named as AGS3, is a receptor-independent G protein activator that has been implicated in multiple biological events such as brain development, neuroplasticity and addiction, cardiac function, Golgi structure/function, macroautophagy and metabolism. It contains seven tetratricopeptide repeats in its N-terminal half and four G-protein regulatory (GPR) motifs in its C-terminal half. It has been shown that AGS3 could regulate the orientation of the mitotic spindle, cAMP production, membrane protein transport, and asymmetric cell division by binding preferentially to inactive Gai/o subunits complexed with guanine dinucleotide phosphate (GDP) at multiple G-protein regulatory or GoLoco motif repeats. It also plays an important anti-apoptotic role through enhancing the phosphorylation of cyclic AMP response element-binding protein (p-CREB).

实验方案

Product Specific Protocols
WB protocol for GPSM1 antibody 68233-1-IgDownload protocol
IF protocol for GPSM1 antibody 68233-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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