验证数据展示
经过测试的应用
Positive WB detected in | LNCaP cells, HeLa cells, HEK-293 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells |
Positive IF/ICC detected in | PC-12 cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:2000-1:10000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
68233-1-Ig targets GPSM1 in WB, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.
经测试应用 | WB, IF/ICC, ELISA Application Description |
经测试反应性 | Human, mouse, rat |
免疫原 | GPSM1 fusion protein Ag18773 种属同源性预测 |
宿主/亚型 | Mouse / IgG1 |
抗体类别 | Monoclonal |
产品类型 | Antibody |
全称 | G-protein signaling modulator 1 (AGS3-like, C. elegans) |
别名 | AGS3, DKFZp727I051, GPSM1 |
计算分子量 | 652 aa, 72 kDa |
观测分子量 | 75 kDa |
GenBank蛋白编号 | BC017353 |
基因名称 | GPSM1 |
Gene ID (NCBI) | 26086 |
RRID | AB_2935320 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein G purification |
UNIPROT ID | Q86YR5 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GPSM1, also named as AGS3, is a receptor-independent G protein activator that has been implicated in multiple biological events such as brain development, neuroplasticity and addiction, cardiac function, Golgi structure/function, macroautophagy and metabolism. It contains seven tetratricopeptide repeats in its N-terminal half and four G-protein regulatory (GPR) motifs in its C-terminal half. It has been shown that AGS3 could regulate the orientation of the mitotic spindle, cAMP production, membrane protein transport, and asymmetric cell division by binding preferentially to inactive Gai/o subunits complexed with guanine dinucleotide phosphate (GDP) at multiple G-protein regulatory or GoLoco motif repeats. It also plays an important anti-apoptotic role through enhancing the phosphorylation of cyclic AMP response element-binding protein (p-CREB).
实验方案
Product Specific Protocols | |
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WB protocol for GPSM1 antibody 68233-1-Ig | Download protocol |
IF protocol for GPSM1 antibody 68233-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |