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验证数据展示

  • WB analysis using 31083-1-AP (same clone as 31083-1-PBS)

    WB analysis using 31083-1-AP (same clone as 31083-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 31083-1-AP (GOLIM4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31083-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 31083-1-AP (same clone as 31083-1-PBS)

    IF Staining of HeLa using 31083-1-AP (same clone as 31083-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GOLIM4 antibody (31083-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 31083-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 31083-1-AP (same clone as 31083-1-PBS)

    WB analysis of HEK-293 using 31083-1-AP (same clone as 31083-1-PBS)

    WB result of GOLIM4 antibody (31083-1-AP; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GOLIM4 transfected HEK-293 cells. This data was developed using the same antibody clone with 31083-1-PBS in a different storage buffer formulation.

  • IHC staining of human intrahepatic cholangiocarcinoma using 31083-1-AP (same clone as 31083-1-PBS)

    IHC staining of human intrahepatic cholangiocarcinoma using 31083-1-AP (same clone as 31083-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 31083-1-AP (GOLIM4 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31083-1-PBS in a different storage buffer formulation.

  • IHC staining of human intrahepatic cholangiocarcinoma using 31083-1-AP (same clone as 31083-1-PBS)

    IHC staining of human intrahepatic cholangiocarcinoma using 31083-1-AP (same clone as 31083-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 31083-1-AP (GOLIM4 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31083-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 31083-1-AP (same clone as 31083-1-PBS)

    IF Staining of HeLa using 31083-1-AP (same clone as 31083-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GOLIM4 antibody (31083-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CoraLite®594-Phalloidin (red). This data was developed using the same antibody clone with 31083-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

GOLIM4 Polyclonal antibody, PBS Only

GOLIM4 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 31083-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

GIMPC, GOLPH4, GPP130, P 138, P138

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

31083-1-PBS targets GOLIM4 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag34962

Product name: Recombinant human GOLIM4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 350-500 aa of NM_014498

Sequence: QVGQAEHLEEEHDPSPEEQDREWKEQHEQREAANLLEGHARAEVYPSAKPMIKFQSPYEEQLEQQRLAVQQVEEAQQLREHQEALHQQRLQGHLLRQQEQQQQQVAREMALQRQAELEEGRPQHQEQLRQQAHYDAMDNDIVQGAEDQGIQ

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 golgi integral membrane protein 4
别名 GIMPC, GOLPH4, GPP130, P 138, P138
计算分子量82 kDa
观测分子量130 kDa
GenBank蛋白编号NM_014498
基因名称 GOLIM4
Gene ID (NCBI) 27333
RRIDAB_3669846
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDO00461
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Golgi integral membrane protein 4 (GOLIM4) is a 130-kDa membrane protein that is integrated into the cis/medial Golgi apparatus, which is a kind II Golgi protein. GOLIM4 is a Golgi protein that circulates between Golgi apparatus and endosomes, and its shuttle is pH sensitive(PMID:30068697).

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