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验证数据展示

  • WB analysis using 31083-1-AP

    WB analysis using 31083-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31083-1-AP (GOLIM4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 31083-1-AP

    IF Staining of HeLa using 31083-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GOLIM4 antibody (31083-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

  • WB analysis of HEK-293 using 31083-1-AP

    WB analysis of HEK-293 using 31083-1-AP

    WB result of GOLIM4 antibody (31083-1-AP; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GOLIM4 transfected HEK-293 cells.

  • IHC staining of human intrahepatic cholangiocarcinoma using 31083-1-AP

    IHC staining of human intrahepatic cholangiocarcinoma using 31083-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 31083-1-AP (GOLIM4 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human intrahepatic cholangiocarcinoma using 31083-1-AP

    IHC staining of human intrahepatic cholangiocarcinoma using 31083-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 31083-1-AP (GOLIM4 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 31083-1-AP

    IF Staining of HeLa using 31083-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GOLIM4 antibody (31083-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CoraLite®594-Phalloidin (red).

  • Featured Product
  • KD/KO Validated

GOLIM4 Polyclonal antibody

GOLIM4 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 31083-1-AP

产品说明书

引用文献(3)

宿主/亚型

Rabbit / IgG

种属反应性

human and More (1)

应用

WB, IHC, IF/ICC, ELISA and More (1)

别名

GIMPC, GOLPH4, GPP130, P 138, P138

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, HepG2 cells
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31083-1-AP targets GOLIM4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IF
经测试反应性 human
文献引用反应性human, mouse
免疫原

CatNo: Ag34962

Product name: Recombinant human GOLIM4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 350-500 aa of NM_014498

Sequence: QVGQAEHLEEEHDPSPEEQDREWKEQHEQREAANLLEGHARAEVYPSAKPMIKFQSPYEEQLEQQRLAVQQVEEAQQLREHQEALHQQRLQGHLLRQQEQQQQQVAREMALQRQAELEEGRPQHQEQLRQQAHYDAMDNDIVQGAEDQGIQ

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 golgi integral membrane protein 4
别名 GIMPC, GOLPH4, GPP130, P 138, P138
计算分子量82 kDa
观测分子量130 kDa
GenBank蛋白编号NM_014498
基因名称 GOLIM4
Gene ID (NCBI) 27333
RRIDAB_3669846
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDO00461
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Golgi integral membrane protein 4 (GOLIM4) is a 130-kDa membrane protein that is integrated into the cis/medial Golgi apparatus, which is a kind II Golgi protein. GOLIM4 is a Golgi protein that circulates between Golgi apparatus and endosomes, and its shuttle is pH sensitive(PMID:30068697).

实验方案

Product Specific Protocols
IF protocol for GOLIM4 antibody 31083-1-APDownload protocol
IHC protocol for GOLIM4 antibody 31083-1-APDownload protocol
WB protocol for GOLIM4 antibody 31083-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle

Cell Death Discov

FBXW7α regulates amyloid pathology by mediating ubiquitination and degradation of BACE1 in Alzheimer's disease.

Authors - Yu Yang
View Article
human,mouseWB,IF

Proc Natl Acad Sci U S A

Monensin suppresses EMT-driven cancer cell motility by inducing Golgi pH-dependent exocytosis of GOLIM4

Authors - Xiaochao Tan
  • KD Validated
View Article
humanWB

Clin Transl Med

PCIF1 drives oesophageal squamous cell carcinoma progression via m6Am-mediated suppression of MTF2 translation

Authors - Kang Li
View Article
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