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验证数据展示

  • WB analysis of HeLa using 66080-1-Ig (same clone as 66080-1-PBS)

    WB analysis of HeLa using 66080-1-Ig (same clone as 66080-1-PBS)

    WB result of GM2A antibody (66080-1-Ig; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GM2A transfected HeLa cells. This data was developed using the same antibody clone with 66080-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 66080-1-Ig (same clone as 66080-1-PBS)

    WB analysis of HeLa using 66080-1-Ig (same clone as 66080-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 66080-1-Ig (GM2A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66080-1-PBS in a different storage buffer formulation.

  • FC experiment of HEK-293 using 66080-1-Ig (same clone as 66080-1-PBS)

    FC experiment of HEK-293 using 66080-1-Ig (same clone as 66080-1-PBS)

    1X10^6 HEK-293 cells were intracellularly stained with 0.4 ug Anti-Human GM2A (66080-1-Ig, Clone:1E4D3) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66080-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

GM2A Monoclonal antibody, PBS Only

GM2A Monoclonal Antibody for WB, FC (Intra), ELISA
Cat No. 66080-1-PBS

产品说明书

CloneNo. 1E4D3

宿主/亚型

Mouse / IgG2a

种属反应性

human

应用

WB, FC (Intra), ELISA

别名

1E4D3, Ganglioside GM2 activator isoform short, GM2AP, GM2-AP, SAP3

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

66080-1-PBS targets GM2A in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 GM2A fusion protein Ag4394 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 GM2 ganglioside activator
别名 1E4D3, Ganglioside GM2 activator isoform short, GM2AP, GM2-AP, SAP3
计算分子量 22 kDa
观测分子量 22 kDa
GenBank蛋白编号BC009273
基因名称 GM2A
Gene ID (NCBI) 2760
RRIDAB_11182593
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP17900
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

GM2A, also named as GM2-AP and SAP-3, is the large binding pocket which can accommodate several single chain phospholipids and fatty acids. GM2A also exhibits some calcium-independent phospholipase activity. It plays a key role in the degradation of ganglioside GM2 (GM2). GM2A stimulates only the breakdown of ganglioside GM2 and glycolipid GA2 by beta-hexosaminidase A.