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验证数据展示

  • WB analysis of HeLa using 66080-1-Ig (same clone as 66080-1-PBS)

    WB analysis of HeLa using 66080-1-Ig (same clone as 66080-1-PBS)

    WB result of GM2A antibody (66080-1-Ig; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GM2A transfected HeLa cells. This data was developed using the same antibody clone with 66080-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 66080-1-Ig (same clone as 66080-1-PBS)

    WB analysis of HeLa using 66080-1-Ig (same clone as 66080-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 66080-1-Ig (GM2A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66080-1-PBS in a different storage buffer formulation.

  • FC experiment of HEK-293 using 66080-1-Ig (same clone as 66080-1-PBS)

    FC experiment of HEK-293 using 66080-1-Ig (same clone as 66080-1-PBS)

    1X10^6 HEK-293 cells were intracellularly stained with 0.4 ug Anti-Human GM2A (66080-1-Ig, Clone:1E4D3) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 66080-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

GM2A Monoclonal antibody, PBS Only

GM2A Monoclonal Antibody for WB, FC (Intra), ELISA
Cat No. 66080-1-PBS

产品说明书

CloneNo. 1E4D3

宿主/亚型

Mouse / IgG2a

种属反应性

human

应用

WB, FC (Intra), ELISA

别名

1E4D3, Ganglioside GM2 activator isoform short, GM2AP, GM2-AP, SAP3

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

66080-1-PBS targets GM2A in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag4394

Product name: Recombinant human GM2A protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-193 aa of BC009273

Sequence: MQSLMQAPLLIALGLLLAAPAQAHLKKPSQLSSFSWDNCDEGKDPAVIRSLTLEPDPIVVPGNVTLSVVGSTSVPLSSPLKVDLVLEKEVAGLWIKIPCTDYIGSCTFEHFCDVLDMLIPTGEPCPEPLRTYGLPCHCPFKEGTYSLPKSEFVVPDLELPSWLTTGNYRIESVLSSSGKRLGCIKIAASLKGI

种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 GM2 ganglioside activator
别名 1E4D3, Ganglioside GM2 activator isoform short, GM2AP, GM2-AP, SAP3
计算分子量 22 kDa
观测分子量 22 kDa
GenBank蛋白编号BC009273
基因名称 GM2A
Gene ID (NCBI) 2760
RRIDAB_11182593
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP17900
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

GM2A, also named as GM2-AP and SAP-3, is the large binding pocket which can accommodate several single chain phospholipids and fatty acids. GM2A also exhibits some calcium-independent phospholipase activity. It plays a key role in the degradation of ganglioside GM2 (GM2). GM2A stimulates only the breakdown of ganglioside GM2 and glycolipid GA2 by beta-hexosaminidase A.

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