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验证数据展示

  • WB analysis of HeLa using 66080-1-Ig

    WB analysis of HeLa using 66080-1-Ig

    WB result of GM2A antibody (66080-1-Ig; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GM2A transfected HeLa cells.

  • WB analysis of HeLa using 66080-1-Ig

    WB analysis of HeLa using 66080-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 66080-1-Ig (GM2A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • FC experiment of HEK-293 using 66080-1-Ig

    FC experiment of HEK-293 using 66080-1-Ig

    1X10^6 HEK-293 cells were intracellularly stained with 0.4 ug Anti-Human GM2A (66080-1-Ig, Clone:1E4D3) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  • Featured Product
  • KD/KO验证

GM2A Monoclonal antibody

GM2A Monoclonal Antibody for WB, FC (Intra), ELISA
Cat No. 66080-1-Ig

产品说明书

CloneNo. 1E4D3

宿主/亚型

Mouse / IgG2a

种属反应性

human

应用

WB, FC (Intra), ELISA

别名

1E4D3, Ganglioside GM2 activator isoform short, GM2AP, GM2-AP, SAP3

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive WB detected inHela cells, human placenta tissue
Positive FC (Intra) detected inHEK-293 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66080-1-Ig targets GM2A in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag4394

Product name: Recombinant human GM2A protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-193 aa of BC009273

Sequence: MQSLMQAPLLIALGLLLAAPAQAHLKKPSQLSSFSWDNCDEGKDPAVIRSLTLEPDPIVVPGNVTLSVVGSTSVPLSSPLKVDLVLEKEVAGLWIKIPCTDYIGSCTFEHFCDVLDMLIPTGEPCPEPLRTYGLPCHCPFKEGTYSLPKSEFVVPDLELPSWLTTGNYRIESVLSSSGKRLGCIKIAASLKGI

种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 GM2 ganglioside activator
别名 1E4D3, Ganglioside GM2 activator isoform short, GM2AP, GM2-AP, SAP3
计算分子量 22 kDa
观测分子量 22 kDa
GenBank蛋白编号BC009273
基因名称 GM2A
Gene ID (NCBI) 2760
RRIDAB_11182593
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP17900
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GM2A, also named as GM2-AP and SAP-3, is the large binding pocket which can accommodate several single chain phospholipids and fatty acids. GM2A also exhibits some calcium-independent phospholipase activity. It plays a key role in the degradation of ganglioside GM2 (GM2). GM2A stimulates only the breakdown of ganglioside GM2 and glycolipid GA2 by beta-hexosaminidase A.

实验方案

Product Specific Protocols
FC protocol for GM2A antibody 66080-1-IgDownload protocol
WB protocol for GM2A antibody 66080-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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