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验证数据展示

  • IF Staining of rat brain using CL532-81063

    IF Staining of rat brain using CL532-81063

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using CoraLite®532 GFAP antibody (CL532-81063, Clone: 4C6 ) at dilution of 1:800. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of rat brain using CL532-81063

    IF Staining of rat brain using CL532-81063

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using CoraLite®532 GFAP antibody (CL532-81063, golden ) at dilution of 1:200, CoraLite®594 NeuN antibody (CL594-66836, red), CoraLite® Plus 488 MBP antibody (CL488-10458, green), CoraLite® Plus 647 MAP2 antibody (CL647-84306-3, cyan). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of rat brain using CL532-81063

    IF Staining of rat brain using CL532-81063

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using CoraLite®532 GFAP antibody (CL532-81063, golden ) at dilution of 1:200, CoraLite®594 NeuN antibody (CL594-66836, red), CoraLite® Plus 488 MBP antibody (CL488-10458, green), CoraLite® Plus 647 MAP2 antibody (CL647-84306-3, cyan). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CoraLite®532-conjugated GFAP Recombinant monoclonal antibody

GFAP Uni-rAb® Recombinant Antibody for IF-P
Cat No. CL532-81063

产品说明书

CloneNo. 4C6

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat, pig

应用

IF-P

别名

4C6, glial fibrillary acidic protein

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®532
规格价格库存

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经过测试的应用

Positive IF-P detected inrat brain tissue
Planning an IHC experiment? We recommend our IHCeasy GFAP Ready-To-Use IHC Kit. GFAP primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 81063-1-PBS

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)-PIF-P : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL532-81063 targets GFAP in IF-P applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 IF-P Application Description
经测试反应性 human, mouse, rat, pig
免疫原

CatNo: Ag10423

Product name: Recombinant human GFAP protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 83-432 aa of BC013596

Sequence: YIEKVRFLEQQNKALAAELNQLRAKEPTKLADVYQAELRELRLRLDQLTANSARLEVERDNLAQDLATVRQKLQDETNLRLEAENNLAAYRQEADEATLARLDLERKIESLEEEIRFLRKIHEEEVRELQEQLARQQVHVELDVAKPDLTAALKEIRTQYEAMASSNMHEAEEWYRSKFADLTDAAARNAELLRQAKHEANDYRRQLQSLTCDLESLRGTNESLERQMREQEERHVREAASYQEALARLEEEGQSLKDEMARHLQEYQDLLNVKLALDIEIATYRKLLEGEENRITIPVQTFSNLQIRETSLDTKSVSEGHLKRNIVVKTVEMRDGEVIKESKQEHKDVM

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 glial fibrillary acidic protein
别名 4C6, glial fibrillary acidic protein
计算分子量 432 aa, 50 kDa
GenBank蛋白编号BC013596
基因名称 GFAP
Gene ID (NCBI) 2670
偶联类型 CoraLite®532 Fluorescent Dye
最大激发/发射波长537 nm / 560 nm
形式Liquid
纯化方式Protein A purification
UNIPROT IDP14136
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GFAP (Glial fibrillary acidic protein ), an intermediate-filament (IF) protein , is specifically expressed in cells of astroglial lineage and is widely used to mark astroglia in the brain. It is also used as a marker for intracranial and intraspinal tumors arising from astrocytes.

实验方案

Product Specific Protocols
IF protocol for CL532 GFAP antibody CL532-81063Download protocol
Standard Protocols
Click here to view our Standard Protocols