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验证数据展示

  • Cytometric bead array sample test of MP80011-1

    Cytometric bead array sample test of MP80011-1

    Cytometric bead array sample test of MP80011-1, GFAP Hybrid Matched Antibody Pair, PBS Only. Capture antibody: 81063-1-PBS. Detection antibody: 60190-4-PBS.

  • WB analysis of U-251 using 81063-1-RR (same clone as 81063-1-PBS)

    WB analysis of U-251 using 81063-1-RR (same clone as 81063-1-PBS)

    U-251 cells were subjected to SDS PAGE followed by western blot with 81063-1-RR (GFAP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81063-1-PBS in a different storage buffer formulation.

  • IHC staining of rat brain using 81063-1-RR (same clone as 81063-1-PBS)

    IHC staining of rat brain using 81063-1-RR (same clone as 81063-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 81063-1-RR (GFAP antibody) at dilution of 1:9900 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81063-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with GFAP antibody included
  • IHC staining of rat brain using 81063-1-RR (same clone as 81063-1-PBS)

    IHC staining of rat brain using 81063-1-RR (same clone as 81063-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 81063-1-RR (GFAP antibody) at dilution of 1:9900 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81063-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with GFAP antibody included
  • IF Staining of rat brain using 81063-1-RR (same clone as 81063-1-PBS)

    IF Staining of rat brain using 81063-1-RR (same clone as 81063-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using GFAP antibody (81063-1-RR, Clone: 4C6 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 81063-1-PBS in a different storage buffer formulation.

  • WB analysis using 81063-1-RR (same clone as 81063-1-PBS)

    WB analysis using 81063-1-RR (same clone as 81063-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 81063-1-RR (GFAP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81063-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse brain using 81063-1-RR (same clone as 81063-1-PBS)

    IF Staining of mouse brain using 81063-1-RR (same clone as 81063-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using GFAP antibody (81063-1-RR, Clone: 4C6 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 81063-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse brain using 81063-1-RR (same clone as 81063-1-PBS)

    IF Staining of mouse brain using 81063-1-RR (same clone as 81063-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using GFAP antibody (81063-1-RR, Clone: 4C6 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 81063-1-PBS in a different storage buffer formulation.

  • WB analysis of U-251 using 81063-1-RR (same clone as 81063-1-PBS)

    WB analysis of U-251 using 81063-1-RR (same clone as 81063-1-PBS)

    WB result of GFAP antibody (81063-1-RR; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GFAP transfected U-251 cells. This data was developed using the same antibody clone with 81063-1-PBS in a different storage buffer formulation.

  • IHC staining of rat brain using 81063-1-PBS

    IHC staining of rat brain using 81063-1-PBS

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 81063-1-PBS (GFAP antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with GFAP antibody included
  • IF Staining of mouse brain using 81063-1-RR (same clone as 81063-1-PBS)

    IF Staining of mouse brain using 81063-1-RR (same clone as 81063-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse brain tissue using GFAP antibody (81063-1-RR, Clone: 4C6 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 81063-1-PBS in a different storage buffer formulation.

  • IF Staining of rat brain using 81063-1-RR (same clone as 81063-1-PBS)

    IF Staining of rat brain using 81063-1-RR (same clone as 81063-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded rat brain tissue using GFAP antibody (81063-1-RR, Clone: 4C6 ) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4), MAP2 antibody (67015-1-Ig, Clone: 1C3E6, green). This data was developed using the same antibody clone with 81063-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP80011-1

    Cytometric bead array standard curve of MP80011-1

    Cytometric bead array standard curve of MP80011-1, GFAP Hybrid Matched Antibody Pair, PBS Only. Capture antibody: 81063-1-PBS. Detection antibody: 60190-4-PBS. Standard:Ag10452. Range: 0.391-400 ng/mL.

  • Affinity and Kinetic Characterization of 81063-1-RR

    Affinity and Kinetic Characterization of 81063-1-RR

    Biolayer interferometry (BLl) kinetic assays of 81077-1-RR against Human GFAP were performed. The affinity constant is below 1 pM.

  • Featured Product
  • KD/KO验证

GFAP Recombinant monoclonal antibody, PBS Only (Capture)

GFAP Uni-rAb® Recombinant Antibody for WB, IHC, IF-P, IF-Fro, Cytometric bead array, Indirect ELISA
Cat No. 81063-1-PBS

产品说明书

CloneNo. 4C6
引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat, pig

应用

WB, IHC, IF-P, IF-Fro, Cytometric bead array, Indirect ELISA

别名

4C6, glial fibrillary acidic protein

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive IHC detected inrat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy GFAP Ready-To-Use IHC Kit. GFAP primary antibody included.

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:5000-1:20000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 1 publications below

产品信息

81063-1-PBS targets GFAP as part of a matched antibody pair:

MP80011-1: 81063-1-PBS capture and 60190-4-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF-P, IF-Fro, Cytometric bead array, Indirect ELISA Application Description
文献引用应用IHC
经测试反应性 human, mouse, rat, pig
文献引用反应性human
免疫原

CatNo: Ag10423

Product name: Recombinant human GFAP protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 83-432 aa of BC013596

Sequence: YIEKVRFLEQQNKALAAELNQLRAKEPTKLADVYQAELRELRLRLDQLTANSARLEVERDNLAQDLATVRQKLQDETNLRLEAENNLAAYRQEADEATLARLDLERKIESLEEEIRFLRKIHEEEVRELQEQLARQQVHVELDVAKPDLTAALKEIRTQYEAMASSNMHEAEEWYRSKFADLTDAAARNAELLRQAKHEANDYRRQLQSLTCDLESLRGTNESLERQMREQEERHVREAASYQEALARLEEEGQSLKDEMARHLQEYQDLLNVKLALDIEIATYRKLLEGEENRITIPVQTFSNLQIRETSLDTKSVSEGHLKRNIVVKTVEMRDGEVIKESKQEHKDVM

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 glial fibrillary acidic protein
别名 4C6, glial fibrillary acidic protein
计算分子量 432 aa, 50 kDa
观测分子量 45-50 kDa
GenBank蛋白编号BC013596
基因名称 GFAP
Gene ID (NCBI) 2670
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP14136
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

GFAP (Glial fibrillary acidic protein ), an intermediate-filament (IF) protein , is specifically expressed in cells of astroglial lineage and is widely used to mark astroglia in the brain. It is also used as a marker for intracranial and intraspinal tumors arising from astrocytes.

发表文章

SpeciesApplicationTitle
humanIHC

Int J Mol Sci

Glial-Cell-Line-Derived Neurotrophic Factor Promotes Glioblastoma Cell Migration and Invasion via the SMAD2/3-SERPINE1-Signaling Axis

Authors - Xiaoxiao Guo
View Article