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验证数据展示

  • WB analysis of U-251 using 81063-1-RR (same clone as 81063-1-PBS)

    WB analysis of U-251 using 81063-1-RR (same clone as 81063-1-PBS)

    U-251 cells were subjected to SDS PAGE followed by western blot with 81063-1-RR (GFAP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81063-1-PBS in a different storage buffer formulation.

  • IHC staining of rat brain using 81063-1-RR (same clone as 81063-1-PBS)

    IHC staining of rat brain using 81063-1-RR (same clone as 81063-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 81063-1-RR (GFAP antibody) at dilution of 1:9900 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81063-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with GFAP antibody included
  • IHC staining of rat brain using 81063-1-RR (same clone as 81063-1-PBS)

    IHC staining of rat brain using 81063-1-RR (same clone as 81063-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 81063-1-RR (GFAP antibody) at dilution of 1:9900 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81063-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with GFAP antibody included
  • IF Staining of rat brain using 81063-1-RR (same clone as 81063-1-PBS)

    IF Staining of rat brain using 81063-1-RR (same clone as 81063-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using GFAP antibody (81063-1-RR, Clone: 4C6 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 81063-1-PBS in a different storage buffer formulation.

  • WB analysis using 81063-1-RR (same clone as 81063-1-PBS)

    WB analysis using 81063-1-RR (same clone as 81063-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 81063-1-RR (GFAP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81063-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse brain using 81063-1-RR (same clone as 81063-1-PBS)

    IF Staining of mouse brain using 81063-1-RR (same clone as 81063-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using GFAP antibody (81063-1-RR, Clone: 4C6 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 81063-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse brain using 81063-1-RR (same clone as 81063-1-PBS)

    IF Staining of mouse brain using 81063-1-RR (same clone as 81063-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using GFAP antibody (81063-1-RR, Clone: 4C6 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 81063-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse brain using 81063-1-RR (same clone as 81063-1-PBS)

    IF Staining of mouse brain using 81063-1-RR (same clone as 81063-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse brain tissue using GFAP antibody (81063-1-RR, Clone: 4C6 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 81063-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 81063-1-RR

    Affinity and Kinetic Characterization of 81063-1-RR

    Biolayer interferometry (BLl) kinetic assays of 81077-1-RR against Human GFAP were performed. The affinity constant is below 1 pM.

GFAP Recombinant antibody, PBS Only

GFAP Uni-rAbTM Recombinant Antibody for WB, IHC, IF-P, IF-Fro, Indirect ELISA
Cat No. 81063-1-PBS

产品说明书

CloneNo. 4C6
引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat, pig

应用

WB, IHC, IF-P, IF-Fro, Indirect ELISA

别名

glial fibrillary acidic protein, 4C6

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy GFAP Ready-To-Use IHC Kit. GFAP primary antibody included.

发表文章中的应用

IHCSee 1 publications below

产品信息

81063-1-PBS targets GFAP in WB, IHC, IF-P, IF-Fro, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, IHC, IF-P, IF-Fro, Indirect ELISA Application Description
文献引用应用IHC
经测试反应性 human, mouse, rat, pig
文献引用反应性human
免疫原 GFAP fusion protein Ag10423 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 glial fibrillary acidic protein
别名 glial fibrillary acidic protein, 4C6
计算分子量 432 aa, 50 kDa
GenBank蛋白编号BC013596
基因名称 GFAP
Gene ID (NCBI) 2670
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP14136
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

GFAP (Glial fibrillary acidic protein ), an intermediate-filament (IF) protein , is specifically expressed in cells of astroglial lineage and is widely used to mark astroglia in the brain. It is also used as a marker for intracranial and intraspinal tumors arising from astrocytes.

发表文章

SpeciesApplicationTitle
humanIHC

Int J Mol Sci

Glial-Cell-Line-Derived Neurotrophic Factor Promotes Glioblastoma Cell Migration and Invasion via the SMAD2/3-SERPINE1-Signaling Axis

Authors - Xiaoxiao Guo
View Article