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验证数据展示

  • FC experiment of HepG2 using CL488-66094

    FC experiment of HepG2 using CL488-66094

    1x10^6 HepG2 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human Fetuin-A (CL488-66094, Clone:1F6B9)(red), or 0.8 ug CoraLite® Plus 488 Mouse IgG2a Isotype Control (C1.18.4) (CL488-65208, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of HepG2 using CL488-66094

    IF Staining of HepG2 using CL488-66094

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 Fetuin-A antibody (CL488-66094, Clone: 1F6B9 ) at dilution of 1:200.

CoraLite® Plus 488-conjugated Fetuin-A/AHSG Monoclonal antibody

Fetuin-A/AHSG Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-66094

产品说明书

CloneNo. 1F6B9

宿主/亚型

Mouse / IgG2a

种属反应性

human

应用

IF/ICC, FC (Intra)

别名

AHSG, Fetuin-A, Alpha 2 Z globulin, alpha 2 HS glycoprotein, AHS

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy AHSG/Fetuin A Ready-To-Use IHC Kit. AHSG/Fetuin A primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66094-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66094 targets Fetuin-A/AHSG in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human
免疫原 Fetuin-A/AHSG fusion protein Ag10073 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 alpha-2-HS-glycoprotein
别名 AHSG, Fetuin-A, Alpha 2 Z globulin, alpha 2 HS glycoprotein, AHS
计算分子量 367 aa, 39 kDa
观测分子量 58 kDa
GenBank蛋白编号BC048198
基因名称 Fetuin-A
Gene ID (NCBI) 197
RRIDAB_2883256
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP02765
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Fetuin-A (alpha-2-HS-glycoprotein, AHSG), a liver borne plasma protein, contributes to the prevention of soft tissue calcification, modulates inflammation, reduces INS sensitivity and fosters weight gain following high fat diet or ageing. It is involved in several functions, such as endocytosis, brain development and the formation of bone tissue. The protein is commonly present in the cortical plate of the immature cerebral cortex and bone marrow hemopoietic matrix, and it has therefore been postulated that it participates in the development of the tissues.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Fetuin-A/AHSG antibody CL488-66094Download protocol
FC protocol for CL Plus 488 Fetuin-A/AHSG antibody CL488-66094Download protocol
Standard Protocols
Click here to view our Standard Protocols