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CoraLite® Plus 488-conjugated FKBP5 Monoclonal antibody

FKBP5 Monoclonal Antibody for FC (Intra), IF/ICC

Host / Isotype

Mouse / IgG2b

Reactivity

Human, rat, mouse

Applications

FC (Intra), IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

3B11F3

Cat No : CL488-67874

Print datasheet

Synonyms

51 kDa FK506 binding protein, 51 kDa FKBP, AIG6, Androgen regulated protein 6, FF1 antigen, FK506 binding protein 5, FKBP 5, FKBP 51, FKBP5, FKBP51, FKBP54, HSP90 binding immunophilin, P54, PPIase, PPIase FKBP5, Ptg 10, Rotamase

验证数据展示

  • FC experiment of K-562 using CL488-67874

    FC experiment of K-562 using CL488-67874

    1X10^6 K-562 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human FKBP5 (CL488-67874, Clone:3B11F3) (red), or 0.4 ug Mouse IgG2b Isotype Control (CL488-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of HeLa using CL488-67874

    IF Staining of HeLa using CL488-67874

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 FKBP5 antibody (CL488-67874, Clone: 3B11F3 ) at dilution of 1:200.

经过测试的应用

Positive IF detected inHeLa cells
Positive FC detected inK-562 cells
Planning an IHC experiment? We recommend our IHCeasy FKBP5 Ready-To-Use IHC Kit. FKBP5 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67874-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67874 targets FKBP5 in IF, FC (Intra) applications and shows reactivity with Human, rat, mouse samples.

Tested Applications FC (Intra), IF/ICC
Tested Reactivity Human, rat, mouse
Immunogen FKBP5 fusion protein Ag5514 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name FK506 binding protein 5
Synonyms 51 kDa FK506 binding protein, 51 kDa FKBP, AIG6, Androgen regulated protein 6, FF1 antigen, FK506 binding protein 5, FKBP 5, FKBP 51, FKBP5, FKBP51, FKBP54, HSP90 binding immunophilin, P54, PPIase, PPIase FKBP5, Ptg 10, Rotamase
Calculated Molecular Weight 457 aa, 51 kDa
Observed Molecular Weight 51 kDa
GenBank Accession NumberBC042605
Gene Symbol FKBP5
Gene ID (NCBI) 2289
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ13451
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FKBP5(Peptidyl-prolyl cis-trans isomerase FKBP5 ) interacts with functionally mature heterooligomeric progesterone receptor complexes along with HSP90 and TEBP. Unlike the predominant T-cell expression of the mouse protein, FKBP5 is expressed as a 51-kD protein in a variety of tissues but not in brain, colon, or lung(PMID: 9125197).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 FKBP5 antibody CL488-67874Download protocol
FC protocol for CL Plus 488 FKBP5 antibody CL488-67874Download protocol
Standard Protocols
Click here to view our Standard Protocols