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FKBP5 Monoclonal antibody

FKBP5 Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, rat, mouse

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

3B11F3

Cat No : 67874-1-Ig

Print datasheet

Synonyms

51 kDa FK506 binding protein, 51 kDa FKBP, AIG6, Androgen regulated protein 6, FF1 antigen, FK506 binding protein 5, FKBP 5, FKBP 51, FKBP5, FKBP51, FKBP54, HSP90 binding immunophilin, P54, PPIase, PPIase FKBP5, Ptg 10, Rotamase

验证数据展示

  • IHC staining of human colon cancer using 67874-1-Ig

    IHC staining of human colon cancer using 67874-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67874-1-Ig (FKBP5 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with FKBP5 antibody included
  • IHC staining of human colon cancer using 67874-1-Ig

    IHC staining of human colon cancer using 67874-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67874-1-Ig (FKBP5 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with FKBP5 antibody included
  • WB analysis using 67874-1-Ig

    WB analysis using 67874-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67874-1-Ig (FKBP5 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

经过测试的应用

Positive WB detected inA549 cells, rat testis tissue, mouse testis tissue, LNCaP cells, HeLa cells, Jurkat cells, K-562 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy FKBP5 Ready-To-Use IHC Kit. FKBP5 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67874-1-Ig targets FKBP5 in WB, IHC, ELISA applications and shows reactivity with Human, rat, mouse samples.

Tested Applications IHC, WB, ELISA
Tested Reactivity Human, rat, mouse
Immunogen FKBP5 fusion protein Ag5514 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name FK506 binding protein 5
Synonyms 51 kDa FK506 binding protein, 51 kDa FKBP, AIG6, Androgen regulated protein 6, FF1 antigen, FK506 binding protein 5, FKBP 5, FKBP 51, FKBP5, FKBP51, FKBP54, HSP90 binding immunophilin, P54, PPIase, PPIase FKBP5, Ptg 10, Rotamase
Calculated Molecular Weight 457 aa, 51 kDa
Observed Molecular Weight 51 kDa
GenBank Accession NumberBC042605
Gene Symbol FKBP5
Gene ID (NCBI) 2289
RRIDAB_2918632
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ13451
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FKBP5(Peptidyl-prolyl cis-trans isomerase FKBP5 ) interacts with functionally mature heterooligomeric progesterone receptor complexes along with HSP90 and TEBP. Unlike the predominant T-cell expression of the mouse protein, FKBP5 is expressed as a 51-kD protein in a variety of tissues but not in brain, colon, or lung(PMID: 9125197).

实验方案

Product Specific Protocols
WB protocol for FKBP5 antibody 67874-1-IgDownload protocol
IHC protocol for FKBP5 antibody 67874-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols