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验证数据展示

  • IF Staining of HeLa using CL488-67712

    IF Staining of HeLa using CL488-67712

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite® Plus 488 EPRS antibody (CL488-67712, Clone: 1B7G2 ) at dilution of 1:200.

CoraLite® Plus 488-conjugated EPRS Monoclonal antibody

EPRS Monoclonal Antibody for IF/ICC
Cat No. CL488-67712

产品说明书

CloneNo. 1B7G2

宿主/亚型

Mouse / IgG2a

种属反应性

Human, rat, mouse

应用

IF/ICC

别名

DKFZp313B047, EARS, EPRS, GLNS, GLUPRORS, GluRS Prolyl tRNA synthetase, Glutamate tRNA ligase, PARS, PIG32, Proline tRNA ligase, QARS, QPRS

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy EPRS Ready-To-Use IHC Kit. EPRS primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67712-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67712 targets EPRS in IF/ICC applications and shows reactivity with Human, rat, mouse samples.

经测试应用 IF/ICC Application Description
经测试反应性 Human, rat, mouse
免疫原 EPRS fusion protein Ag19184 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 glutamyl-prolyl-tRNA synthetase
别名 DKFZp313B047, EARS, EPRS, GLNS, GLUPRORS, GluRS Prolyl tRNA synthetase, Glutamate tRNA ligase, PARS, PIG32, Proline tRNA ligase, QARS, QPRS
计算分子量 1512 aa, 171 kDa
GenBank蛋白编号BC126275
基因名称 EPRS
Gene ID (NCBI) 2058
RRIDAB_2919526
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP07814
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Human EPRS is a 172 kDa, 1512 amino acid polypeptide consisting of three major domains. The N and C termini contain ERS and PRS catalytic domains, respectively, joined by a 300 amino acid linker containing three tandem WHEP-TRS (referred to as WHEP) domains. EPRS is a bifunctional aminoacyl-tRNA synthetase that catalyzes the aminoacylation of glutamic acid and proline tRNA species. EPRS has a special role in GAIT-mediated translational control, as it is solely responsible for recognition and interaction with GAIT elements in target mRNAs. (PMID: 29576217, PMID: 22386318, PMID: 19647514)

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 EPRS antibody CL488-67712Download protocol
Standard Protocols
Click here to view our Standard Protocols