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EPRS Monoclonal antibody

EPRS Monoclonal Antibody for IF/ICC, IHC, WB,ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, rat, mouse and More (1)

Applications

IF/ICC, IHC, WB,ELISA

Conjugate

Unconjugated

CloneNo.

1B7G2

Cat No : 67712-1-Ig

Print datasheet

Synonyms

DKFZp313B047, EARS, EPRS, GLNS, GLUPRORS, GluRS Prolyl tRNA synthetase, Glutamate tRNA ligase, PARS, PIG32, Proline tRNA ligase, QARS, QPRS

验证数据展示

  • WB analysis using 67712-1-Ig

    WB analysis using 67712-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67712-1-Ig (EPRS antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 67712-1-Ig

    IHC staining of human lung cancer using 67712-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67712-1-Ig (EPRS antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with EPRS antibody included
  • IF Staining of HeLa using 67712-1-Ig

    IF Staining of HeLa using 67712-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using EPRS antibody (67712-1-Ig, Clone: 1B7G2 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • IF Staining of HeLa using 67712-1-Ig

    IF Staining of HeLa using 67712-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using EPRS antibody (67712-1-Ig, Clone: 1B7G2 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). DAPI (blue).

经过测试的应用

Positive WB detected inHT-29 cells, HSC-T6 cells, NIH/3T3 cells, PC-12 cells, HeLa cells, HEK-293 cells, HepG2 cells, LNCaP cells, Jurkat cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy EPRS Ready-To-Use IHC Kit. EPRS primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite® Plus 647 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67712-1-Ig targets EPRS in WB, IF, IHC, ELISA applications and shows reactivity with Human, rat, mouse samples.

Tested Applications IF/ICC, IHC, WB,ELISA
Cited ApplicationsWB, IF, IHC
Tested Reactivity Human, rat, mouse
Cited Reactivityhuman, mouse
Immunogen EPRS fusion protein Ag19184 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name glutamyl-prolyl-tRNA synthetase
Synonyms DKFZp313B047, EARS, EPRS, GLNS, GLUPRORS, GluRS Prolyl tRNA synthetase, Glutamate tRNA ligase, PARS, PIG32, Proline tRNA ligase, QARS, QPRS
Calculated Molecular Weight 1512 aa, 171 kDa
Observed Molecular Weight171 kDa
GenBank Accession NumberBC126275
Gene Symbol EPRS
Gene ID (NCBI) 2058
RRIDAB_2882902
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP07814
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

Human EPRS is a 172 kDa, 1512 amino acid polypeptide consisting of three major domains. The N and C termini contain ERS and PRS catalytic domains, respectively, joined by a 300 amino acid linker containing three tandem WHEP-TRS (referred to as WHEP) domains. EPRS is a bifunctional aminoacyl-tRNA synthetase that catalyzes the aminoacylation of glutamic acid and proline tRNA species. EPRS has a special role in GAIT-mediated translational control, as it is solely responsible for recognition and interaction with GAIT elements in target mRNAs. (PMID: 29576217, PMID: 22386318, PMID: 19647514)

实验方案

Product Specific Protocols
WB protocol for EPRS antibody 67712-1-IgDownload protocol
IHC protocol for EPRS antibody 67712-1-IgDownload protocol
IF protocol for EPRS antibody 67712-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,mouseWB,IF,IHC

Kaohsiung J Med Sci

Evodiamine inhibits EPRS expression to regulate glutamate metabolism and proliferation of oral squamous cell carcinoma cells

Authors - Li-Qi Lin
View Article