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Host
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Species Reactivity
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Species Reactivity
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Conjugate
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Compatible Species
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Conjugate
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验证数据展示

  • WB analysis using 67712-1-Ig (same clone as 67712-1-PBS)

    WB analysis using 67712-1-Ig (same clone as 67712-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67712-1-Ig (EPRS antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67712-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 67712-1-Ig (same clone as 67712-1-PBS)

    IHC staining of human lung cancer using 67712-1-Ig (same clone as 67712-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67712-1-Ig (EPRS antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67712-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with EPRS antibody included
  • IF Staining of HeLa using 67712-1-Ig (same clone as 67712-1-PBS)

    IF Staining of HeLa using 67712-1-Ig (same clone as 67712-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using EPRS antibody (67712-1-Ig, Clone: 1B7G2 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67712-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 67712-1-Ig (same clone as 67712-1-PBS)

    IF Staining of HeLa using 67712-1-Ig (same clone as 67712-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using EPRS antibody (67712-1-Ig, Clone: 1B7G2 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). DAPI (blue). This data was developed using the same antibody clone with 67712-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 67712-1-Ig (same clone as 67712-1-PBS)

    IP experiment of HeLa using 67712-1-Ig (same clone as 67712-1-PBS)

    IP result of anti-EPRS (IP:67712-1-Ig, 4ug; Detection:67712-1-Ig 1:10000) with HeLa cells lysate 1085 ug. This data was developed using the same antibody clone with 67712-1-PBS in a different storage buffer formulation.

  • IF Staining of BV-2 using 67712-1-Ig (same clone as 67712-1-PBS)

    IF Staining of BV-2 using 67712-1-Ig (same clone as 67712-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed BV-2 cells using EPRS antibody (67712-1-Ig, Clone: 1B7G2 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 67712-1-PBS in a different storage buffer formulation.

EPRS Monoclonal antibody, PBS Only

EPRS Monoclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 67712-1-PBS

产品说明书

CloneNo. 1B7G2

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

EPRS1, EARS, Cell proliferation-inducing gene 32 protein, Bifunctional glutamate/proline--tRNA ligase, Bifunctional aminoacyl-tRNA synthetase

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy EPRS Ready-To-Use IHC Kit. EPRS primary antibody included.

产品信息

67712-1-PBS targets EPRS in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag19184

Product name: Recombinant human EPRS protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1163-1512 aa of BC126275

Sequence: RTREFLWQEGHSAFATMEEAAEEVLQILDLYAQVYEELLAIPVVKGRKTEKEKFAGGDYTTTIEAFISASGRAIQGGTSHHLGQNFSKMFEIVFEDPKIPGEKQFAYQNSWGLTTRTIGVMTMVHGDNMGLVLPPRVACVQVVIIPCGITNALSEEDKEALIAKCNDYRRRLLSVNIRVRADLRDNYSPGWKFNHWELKGVPIRLEVGPRDMKSCQFVAVRRDTGEKLTVAENEAETKLQAILEDIQVTLFTRASEDLKTHMVVANTMEDFQKILDSGKIVQIPFCGEIDCEDWIKKTTARDQDLEPGAPSMGAKSLCIPFKPLCELQPGAKCVCGKNPAKYYTLFGRSY

种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 glutamyl-prolyl-tRNA synthetase
别名 EPRS1, EARS, Cell proliferation-inducing gene 32 protein, Bifunctional glutamate/proline--tRNA ligase, Bifunctional aminoacyl-tRNA synthetase
计算分子量 1512 aa, 171 kDa
观测分子量171 kDa
GenBank蛋白编号BC126275
基因名称 EPRS
Gene ID (NCBI) 2058
RRIDAB_2882902
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP07814
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Human EPRS is a 172 kDa, 1512 amino acid polypeptide consisting of three major domains. The N and C termini contain ERS and PRS catalytic domains, respectively, joined by a 300 amino acid linker containing three tandem WHEP-TRS (referred to as WHEP) domains. EPRS is a bifunctional aminoacyl-tRNA synthetase that catalyzes the aminoacylation of glutamic acid and proline tRNA species. EPRS has a special role in GAIT-mediated translational control, as it is solely responsible for recognition and interaction with GAIT elements in target mRNAs. (PMID: 29576217, PMID: 22386318, PMID: 19647514)