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验证数据展示

  • IF Staining of A549 using FITC-67322

    IF Staining of A549 using FITC-67322

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using FITC Plus EPHX2 antibody (FITC-67322, Clone: 4H6C8 ) at dilution of 1:50.

  • Featured Product
  • KD/KO验证

FITC Plus-conjugated EPHX2 Monoclonal antibody

EPHX2 Monoclonal Antibody for IF/ICC
Cat No. FITC-67322

产品说明书

CloneNo. 4H6C8

宿主/亚型

Mouse / IgG2b

种属反应性

Human, Pig

应用

IF/ICC

别名

SEH, Lipid-phosphate phosphatase, Cytosolic epoxide hydrolase 2, CEH, Bifunctional epoxide hydrolase 2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  FITC Plus
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inA549 cells
Planning an IHC experiment? We recommend our IHCeasy EPHX2 Ready-To-Use IHC Kit. EPHX2 primary antibody included.

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

FITC-67322 targets EPHX2 in IF/ICC applications and shows reactivity with Human, Pig samples.

经测试应用 IF/ICC Application Description
经测试反应性 Human, Pig
免疫原 EPHX2 fusion protein Ag4870 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 epoxide hydrolase 2, cytoplasmic
别名 SEH, Lipid-phosphate phosphatase, Cytosolic epoxide hydrolase 2, CEH, Bifunctional epoxide hydrolase 2
计算分子量 63 kDa
观测分子量 56-63 kDa
GenBank蛋白编号BC013874
基因名称 EPHX2
Gene ID (NCBI) 2053
RRIDAB_3673875
偶联类型 FITC Plus Fluorescent Dye
最大激发/发射波长495 nm / 524 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP34913
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

EPHX2(Epoxide hydrolase 2) acts on epoxides (alkene oxides, oxiranes) and arene oxides and plays a role in xenobiotic metabolism by degrading potentially toxic epoxides. A number of single nucleotide polymorphisms (SNPs) in human EPHX2 have been linked to cardiovascular disease risk, including increased risk of coronary heart disease, hyperlipoproteinemia, and type-2 diabetes (PMID: 14732757, 16595607, 14673705, 15845398, 17460077). It was observed in many tissues with the band of 63 kDa in the western blot. It has also been reported that the N-terminal domain might promote dimerization of EPHX2 (PMID:21553642).

实验方案

Product Specific Protocols
IF protocol for FITC Plus EPHX2 antibody FITC-67322Download protocol
Standard Protocols
Click here to view our Standard Protocols