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EPHX2 Monoclonal antibody

EPHX2 Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Pig

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

4H6C8

Cat No : 67322-1-Ig

Print datasheet

Synonyms

CEH, Cytosolic epoxide hydrolase, EPHX2, Epoxide hydratase, Epoxide hydrolase 2, SEH, Soluble epoxide hydrolase

验证数据展示

  • WB analysis using 67322-1-Ig

    WB analysis using 67322-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67322-1-Ig (EPHX2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of A549 using 67322-1-Ig

    WB analysis of A549 using 67322-1-Ig

    WB result of EPHX2 antibody (67322-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EPHX2 transfected A549 cells.

  • IHC staining of human breast cancer using 67322-1-Ig

    IHC staining of human breast cancer using 67322-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67322-1-Ig (EPHX2 antibody) at dilution of 1:4000 (under 10x lens).

    Ready-to-use IHC kits available with EPHX2 antibody included
  • IHC staining of human breast cancer using 67322-1-Ig

    IHC staining of human breast cancer using 67322-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67322-1-Ig (EPHX2 antibody) at dilution of 1:4000 (under 40x lens).

    Ready-to-use IHC kits available with EPHX2 antibody included

经过测试的应用

Positive WB detected inLNCaP cells, A549 cells, Jurkat cells, pig liver tissue
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy EPHX2 Ready-To-Use IHC Kit. EPHX2 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67322-1-Ig targets EPHX2 in WB, IHC, ELISA applications and shows reactivity with Human, Pig samples.

Tested Applications IHC, WB, ELISA
Tested Reactivity Human, Pig
Immunogen EPHX2 fusion protein Ag4870 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name epoxide hydrolase 2, cytoplasmic
Synonyms CEH, Cytosolic epoxide hydrolase, EPHX2, Epoxide hydratase, Epoxide hydrolase 2, SEH, Soluble epoxide hydrolase
Calculated Molecular Weight 63 kDa
Observed Molecular Weight 56-63 kDa
GenBank Accession NumberBC013874
Gene Symbol EPHX2
Gene ID (NCBI) 2053
RRIDAB_2882582
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP34913
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

EPHX2(Epoxide hydrolase 2) acts on epoxides (alkene oxides, oxiranes) and arene oxides and plays a role in xenobiotic metabolism by degrading potentially toxic epoxides. A number of single nucleotide polymorphisms (SNPs) in human EPHX2 have been linked to cardiovascular disease risk, including increased risk of coronary heart disease, hyperlipoproteinemia, and type-2 diabetes (PMID: 14732757, 16595607, 14673705, 15845398, 17460077). It was observed in many tissues with the band of 63 kDa in the western blot. It has also been reported that the N-terminal domain might promote dimerization of EPHX2 (PMID:21553642).

实验方案

Product Specific Protocols
WB protocol for EPHX2 antibody 67322-1-IgDownload protocol
IHC protocol for EPHX2 antibody 67322-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols