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CoraLite® Plus 488-conjugated ELA2 Polyclonal antibody

ELA2 Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat No : CL488-27642

Print datasheet

Synonyms

Bone marrow serine protease, ELA2, ELANE, Elastase 2, elastase 2, neutrophil, GE, HLE, HNE, Human leukocyte elastase, Medullasin, NE, Neutrophil elastase, PMN E, PMN elastase

验证数据展示

  • IF Staining of HEK-293 using CL488-27642

    IF Staining of HEK-293 using CL488-27642

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using CoraLite® Plus 488 ELA2 antibody (CL488-27642) at dilution of 1:100.

经过测试的应用

Positive IF detected inHEK-293 cells
Planning an IHC experiment? We recommend our IHCeasy ELANE/ELA2 Ready-To-Use IHC Kit. ELANE/ELA2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 27642-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-27642 targets ELA2 in IF applications and shows reactivity with Human samples.

Tested Applications IF/ICC
Tested Reactivity Human
Immunogen ELA2 fusion protein Ag26634 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name elastase 2, neutrophil
Synonyms Bone marrow serine protease, ELA2, ELANE, Elastase 2, elastase 2, neutrophil, GE, HLE, HNE, Human leukocyte elastase, Medullasin, NE, Neutrophil elastase, PMN E, PMN elastase
Calculated Molecular Weight 267 aa, 29 kDa
Observed Molecular Weight29 kDa
GenBank Accession NumberBC074816
Gene Symbol ELA2
Gene ID (NCBI) 1991
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP08246
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ELA2(Neutrophil elastase) is also named as ELAL, NE, HLE, HNE, PMN-E, SERP1, GE, ELANE and belongs to the peptidase S1 family. It is a 33-kDa enzyme with several isoforms that differ in their extent of glycosylation(PMID:12223222). Human neutrophil elastase (HNE) is a serine protease with potent proteolytic activity (3), which is reported to cause mucin secretion (degranulation)(PMID:12169572). It may contribute to the directed migration of leukocytes into flamed postcapillary venules in addition to contributing to the process of tissue emigration(PMID:9683424). The full length protein has a signal peptide,propeptide and three glycosylation sites.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 ELA2 antibody CL488-27642Download protocol
Standard Protocols
Click here to view our Standard Protocols