ELA2 Polyclonal antibody
ELA2 Polyclonal Antibody for IF/ICC, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human, mouse and More (2)
Applications
IF/ICC, IHC, WB, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | THP-1 cells, U-937 cells |
Positive IHC detected in | human lymphoma tissue, mouse spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HEK-293 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
27642-1-AP targets ELA2 in WB, IF, IHC, ELISA applications and shows reactivity with Human, mouse samples.
Tested Applications | IF/ICC, IHC, WB, ELISA |
Cited Applications | WB, IF, IHC |
Tested Reactivity | Human, mouse |
Cited Reactivity | mouse, rat, bovine |
Immunogen | ELA2 fusion protein Ag26634 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | elastase 2, neutrophil |
Synonyms | Bone marrow serine protease, ELA2, ELANE, Elastase 2, elastase 2, neutrophil, GE, HLE, HNE, Human leukocyte elastase, Medullasin, NE, Neutrophil elastase, PMN E, PMN elastase |
Calculated Molecular Weight | 267 aa, 29 kDa |
Observed Molecular Weight | 29 kDa |
GenBank Accession Number | BC074816 |
Gene Symbol | ELA2 |
Gene ID (NCBI) | 1991 |
RRID | AB_2918126 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P08246 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for ELA2 antibody 27642-1-AP | Download protocol |
IHC protocol for ELA2 antibody 27642-1-AP | Download protocol |
IF protocol for ELA2 antibody 27642-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Cell Mol Biol Lett Obacunone alleviates ferroptosis during lipopolysaccharide-induced acute lung injury by upregulating Nrf2-dependent antioxidant responses. | ||
Animals (Basel) Effects of Hormone, NEFA and SCFA on the Migration of Neutrophils and the Formation of Neutrophil Extracellular Traps in Dairy Cows. | ||
Cancer Immunol Res Tumor cell-derived microparticles induced by methotrexate augment T-cell antitumor responses by down-regulating expression of PD-1 in neutrophils | ||
Front Pharmacol RNA-sequencing approach for exploring the protective mechanisms of dexmedetomidine on pancreatic injury in severe acute pancreatitis |