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验证数据展示

  • WB analysis using 67855-1-Ig

    WB analysis using 67855-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67855-1-Ig (EFTUD2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control.

  • IHC staining of human liver cancer using 67855-1-Ig

    IHC staining of human liver cancer using 67855-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67855-1-Ig (EFTUD2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 67855-1-Ig

    IF Staining of HeLa using 67855-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using EFTUD2 antibody (67855-1-Ig, Clone: 2F4G2 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • WB analysis of HeLa using 67855-1-Ig

    WB analysis of HeLa using 67855-1-Ig

    WB result of EFTUD2 antibody (67855-1-Ig; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EFTUD2 transfected HeLa cells.

  • Featured Product
  • KD/KO Validated

EFTUD2 Monoclonal antibody

EFTUD2 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 67855-1-Ig

产品说明书

CloneNo. 2F4G2
引用文献(2)

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

116 kDa U5 small nuclear ribonucleoprotein component, 2F4G2, Elongation factor Tu GTP-binding domain-containing protein 2, hSNU114, KIAA0031

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive WB detected inLNCaP cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, PC-12 cells, NIH/3T3 cells, RAW 264.7 cells
Positive IHC detected inhuman liver cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

67855-1-Ig targets EFTUD2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原

CatNo: Ag24345

Product name: Recombinant human EFTUD2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 13-217 aa of BC002360

Sequence: IGPELDSDEDDDELGRETKDLDEMDDDDDDDDVGDHDDDHPGMEVVLHEDKKYYPTAEEVYGPEVETIVQEEDTQPLTEPIIKPVKTKKFTLMEQTLPVTVYEMDFLADLMDNSELIRNVTLCGHLHHGKTCFVDCLIEQTHPEIRKRYDQDLCYTDILFTEQERGVGIKSTPVTVVLPDTKGKSYLFNIMDTPGHVNFSDEVTA

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 elongation factor Tu GTP binding domain containing 2
别名 116 kDa U5 small nuclear ribonucleoprotein component, 2F4G2, Elongation factor Tu GTP-binding domain-containing protein 2, hSNU114, KIAA0031
计算分子量 116 kDa
观测分子量 109-120 kDa
GenBank蛋白编号BC002360
基因名称 EFTUD2
Gene ID (NCBI) 9343
RRIDAB_2918613
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ15029
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for EFTUD2 antibody 67855-1-IgDownload protocol
IHC protocol for EFTUD2 antibody 67855-1-IgDownload protocol
WB protocol for EFTUD2 antibody 67855-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Exp Clin Cancer Res

The feedback loop of EFTUD2/c-MYC impedes chemotherapeutic efficacy by enhancing EFTUD2 transcription and stabilizing c-MYC protein in colorectal cancer

Authors - Xiaojian Zhu
View Article

Br J Cancer

Spliceosomal GTPase EFTUD2 mediates DDX41 intron retention to promote the malignant progression of ovarian cancer

Authors - Yanling Liu
View Article
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