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EFTUD2 Monoclonal antibody

EFTUD2 Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat and More (1)

Applications

IF, IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

2F4G2

Cat No : 67855-1-Ig

Print datasheet

Synonyms

DKFZp686E24196, EFTUD2, FLJ44695, hSNU114, KIAA0031, Snrp116, Snu114, SNU114 homolog, U5 116 kDa, U5 116KD

验证数据展示

  • WB analysis using 67855-1-Ig

    WB analysis using 67855-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67855-1-Ig (EFTUD2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control.

  • IHC staining of human liver cancer using 67855-1-Ig

    IHC staining of human liver cancer using 67855-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67855-1-Ig (EFTUD2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 67855-1-Ig

    IF Staining of HeLa using 67855-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using EFTUD2 antibody (67855-1-Ig, Clone: 2F4G2 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • WB analysis of HeLa using 67855-1-Ig

    WB analysis of HeLa using 67855-1-Ig

    WB result of EFTUD2 antibody (67855-1-Ig; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EFTUD2 transfected HeLa cells.

经过测试的应用

Positive WB detected inLNCaP cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, PC-12 cells, NIH/3T3 cells, RAW 264.7 cells
Positive IHC detected inhuman liver cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

67855-1-Ig targets EFTUD2 in WB, IF, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications IF, IHC, WB, ELISA
Cited ApplicationsWB
Tested Reactivity Human, mouse, rat
Cited Reactivityhuman
Immunogen EFTUD2 fusion protein Ag24345 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name elongation factor Tu GTP binding domain containing 2
Synonyms DKFZp686E24196, EFTUD2, FLJ44695, hSNU114, KIAA0031, Snrp116, Snu114, SNU114 homolog, U5 116 kDa, U5 116KD
Calculated Molecular Weight 116 kDa
Observed Molecular Weight 109-120 kDa
GenBank Accession NumberBC002360
Gene Symbol EFTUD2
Gene ID (NCBI) 9343
RRIDAB_2918613
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ15029
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for EFTUD2 antibody 67855-1-IgDownload protocol
IHC protocol for EFTUD2 antibody 67855-1-IgDownload protocol
IF protocol for EFTUD2 antibody 67855-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Exp Clin Cancer Res

The feedback loop of EFTUD2/c-MYC impedes chemotherapeutic efficacy by enhancing EFTUD2 transcription and stabilizing c-MYC protein in colorectal cancer

Authors - Xiaojian Zhu
View Article