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验证数据展示

  • WB analysis using 67855-1-Ig (same clone as 67855-1-PBS)

    WB analysis using 67855-1-Ig (same clone as 67855-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67855-1-Ig (EFTUD2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 67855-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 67855-1-Ig (same clone as 67855-1-PBS)

    IHC staining of human liver cancer using 67855-1-Ig (same clone as 67855-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67855-1-Ig (EFTUD2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67855-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 67855-1-Ig (same clone as 67855-1-PBS)

    IF Staining of HeLa using 67855-1-Ig (same clone as 67855-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using EFTUD2 antibody (67855-1-Ig, Clone: 2F4G2 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67855-1-PBS in a different storage buffer formulation.

  • Cytometric bead array sample test of MP51817-1

    Cytometric bead array sample test of MP51817-1

    Cytometric bead array sample test of MP51817-1, EFTUD2 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67855-1-PBS. Detection antibody: 67855-2-PBS.

  • WB analysis of HeLa using 67855-1-Ig (same clone as 67855-1-PBS)

    WB analysis of HeLa using 67855-1-Ig (same clone as 67855-1-PBS)

    WB result of EFTUD2 antibody (67855-1-Ig; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EFTUD2 transfected HeLa cells. This data was developed using the same antibody clone with 67855-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP51817-1

    Cytometric bead array standard curve of MP51817-1

    Cytometric bead array standard curve of MP51817-1, EFTUD2 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67855-1-PBS. Detection antibody: 67855-2-PBS. Standard:Ag24345. Range: 0.781-50 ng/mL.

  • Featured Product
  • KD/KO Validated

EFTUD2 Monoclonal antibody, PBS Only (Capture)

EFTUD2 Monoclonal Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 67855-1-PBS

产品说明书

CloneNo. 2F4G2

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

别名

116 kDa U5 small nuclear ribonucleoprotein component, 2F4G2, Elongation factor Tu GTP-binding domain-containing protein 2, hSNU114, KIAA0031

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

67855-1-PBS targets EFTUD2 as part of a matched antibody pair:

MP51817-1: 67855-1-PBS capture and 67855-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag24345

Product name: Recombinant human EFTUD2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 13-217 aa of BC002360

Sequence: IGPELDSDEDDDELGRETKDLDEMDDDDDDDDVGDHDDDHPGMEVVLHEDKKYYPTAEEVYGPEVETIVQEEDTQPLTEPIIKPVKTKKFTLMEQTLPVTVYEMDFLADLMDNSELIRNVTLCGHLHHGKTCFVDCLIEQTHPEIRKRYDQDLCYTDILFTEQERGVGIKSTPVTVVLPDTKGKSYLFNIMDTPGHVNFSDEVTA

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 elongation factor Tu GTP binding domain containing 2
别名 116 kDa U5 small nuclear ribonucleoprotein component, 2F4G2, Elongation factor Tu GTP-binding domain-containing protein 2, hSNU114, KIAA0031
计算分子量 116 kDa
观测分子量 109-120 kDa
GenBank蛋白编号BC002360
基因名称 EFTUD2
Gene ID (NCBI) 9343
RRIDAB_2918613
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ15029
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C
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