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验证数据展示

  • FC experiment of HepG2 using CL488-67855

    FC experiment of HepG2 using CL488-67855

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human EFTUD2 (CL488-67855, Clone:2F4G2) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated EFTUD2 Monoclonal antibody

EFTUD2 Monoclonal Antibody for FC (Intra)
Cat No. CL488-67855

产品说明书

CloneNo. 2F4G2

宿主/亚型

Mouse / IgG1

种属反应性

Human, mouse, rat

应用

FC (Intra)

别名

DKFZp686E24196, EFTUD2, FLJ44695, hSNU114, KIAA0031, Snrp116, Snu114, SNU114 homolog, U5 116 kDa, U5 116KD

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive FC (Intra) detected inHepG2 cells
Positive FC detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 67855-1-Ig

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67855 targets EFTUD2 in FC (Intra) applications and shows reactivity with Human, mouse, rat samples.

经测试应用 FC (Intra) Application Description
经测试反应性 Human, mouse, rat
免疫原

CatNo: Ag24345

Product name: Recombinant human EFTUD2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 13-217 aa of BC002360

Sequence: IGPELDSDEDDDELGRETKDLDEMDDDDDDDDVGDHDDDHPGMEVVLHEDKKYYPTAEEVYGPEVETIVQEEDTQPLTEPIIKPVKTKKFTLMEQTLPVTVYEMDFLADLMDNSELIRNVTLCGHLHHGKTCFVDCLIEQTHPEIRKRYDQDLCYTDILFTEQERGVGIKSTPVTVVLPDTKGKSYLFNIMDTPGHVNFSDEVTA

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 elongation factor Tu GTP binding domain containing 2
别名 DKFZp686E24196, EFTUD2, FLJ44695, hSNU114, KIAA0031, Snrp116, Snu114, SNU114 homolog, U5 116 kDa, U5 116KD
计算分子量 116 kDa
观测分子量 109-120 kDa
GenBank蛋白编号BC002360
基因名称 EFTUD2
Gene ID (NCBI) 9343
RRIDAB_2923804
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ15029
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 EFTUD2 antibody CL488-67855Download protocol
Standard Protocols
Click here to view our Standard Protocols