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验证数据展示

  • IF Staining of HeLa using CL488-82887

    IF Staining of HeLa using CL488-82887

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 E2F7 antibody (CL488-82887, Clone: 230073C2 ) at dilution of 1:200.

  • FC experiment of HeLa using CL488-82887

    FC experiment of HeLa using CL488-82887

    1x10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488-conjugated E2F7 Recombinant antibody (CL488-82887, Clone:230073C2)(red), or 0.4 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

CoraLite® Plus 488-conjugated E2F7 Recombinant monoclonal antibody

E2F7 Uni-rAb® Recombinant Antibody for IF/ICC, FC (Intra)
Cat No. CL488-82887

产品说明书

CloneNo. 230073C2

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, FC (Intra)

别名

Transcription factor E2F7, E2F-7, 230073C2

缓冲液配方:  PBS, Proclin 300, BSA, Glycerol
PBS, Proclin 300, BSA, Glycerol
偶联物:  CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 82887-1-PBS

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-82887 targets E2F7 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human
免疫原

CatNo: Ag21599

Product name: Recombinant human E2F7 protein

Source: e coli.-derived, PET30a

Tag: 6*His

Domain: 369-713 aa of BC136366

Sequence: VDFSSSDEELVDVSASVLPELKRETYGQIQVCAKQKLARHGSFNTVQASERIQRKVNSEPSSPYREEQGSGGYSLEIGSLAAVYRQKIEDNSQGKAFASKRVVPPSSSLDPVAPFPVLSVDPEYCVNPLAHPVFSVAQTDLQAFSMQNGLNGQVDVSLASAASAVESLKPALLAGQPLVYVPSASLFMLYGSLQEGPASGSGSERDDRSSEAPATVELSSAPSAQKRLCEERKPQEEDEPATKRQSREYEDGPLSLVMPKKPSDSTDLASPKTMGNRASIPLKDIHVNGQLPAAEEISGKATANSLVSSEWGNPSRNTDVEKPSKENESTKEPSLLQYLCVQSPA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 E2F transcription factor 7
别名 Transcription factor E2F7, E2F-7, 230073C2
计算分子量 911 aa, 100 kDa
GenBank蛋白编号BC136366
基因名称 E2F7
Gene ID (NCBI) 144455
RRIDAB_3673097
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ96AV8
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

E2F7 also named as E2F transcription factor 7 is a 911 amino acid protein, which belongs to the E2F/DP family. E2F7 localizes in the nucleus and mainly forms homodimers and, to a lesser extent, heterodimers with E2F8. E2F7 as a transcription factor involves in various processes such as angiogenesis, polyploidization of specialized cells and DNA damage response. E2F7 mainly forms homodimers and to a lesser extent, heterodimers with E2F8. (PMID: 18194653 )

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 E2F7 antibody CL488-82887Download protocol
IF protocol for CL Plus 488 E2F7 antibody CL488-82887Download protocol
Standard Protocols
Click here to view our Standard Protocols
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