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验证数据展示

  • IF Staining of HeLa using 82887-1-RR (same clone as 82887-1-PBS)

    IF Staining of HeLa using 82887-1-RR (same clone as 82887-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using E2F7 antibody (82887-1-RR, Clone: 230073C2 ) at dilution of 1:1000 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR004).(CL488-Phalloidin, green). This data was developed using the same antibody clone with 82887-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 82887-1-RR (same clone as 82887-1-PBS)

    FC experiment of HeLa using 82887-1-RR (same clone as 82887-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug E2F7 Recombinant antibody (82887-1-RR, Clone:230073C2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 82887-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82887-1-RR

    Affinity and Kinetic Characterization of 82887-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82887-1-RR against Human E2F7 were performed. The affinity constant is 31.3 nM.

E2F7 Recombinant monoclonal antibody, PBS Only

E2F7 Uni-rAb® Recombinant Antibody for IF/ICC, FC (Intra), Indirect ELISA
Cat No. 82887-1-PBS

产品说明书

CloneNo. 230073C2

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, FC (Intra), Indirect ELISA

别名

230073C2, E2F-7, Transcription factor E2F7

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

82887-1-PBS targets E2F7 in IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag21599

Product name: Recombinant human E2F7 protein

Source: e coli.-derived, PET30a

Tag: 6*His

Domain: 369-713 aa of BC136366

Sequence: VDFSSSDEELVDVSASVLPELKRETYGQIQVCAKQKLARHGSFNTVQASERIQRKVNSEPSSPYREEQGSGGYSLEIGSLAAVYRQKIEDNSQGKAFASKRVVPPSSSLDPVAPFPVLSVDPEYCVNPLAHPVFSVAQTDLQAFSMQNGLNGQVDVSLASAASAVESLKPALLAGQPLVYVPSASLFMLYGSLQEGPASGSGSERDDRSSEAPATVELSSAPSAQKRLCEERKPQEEDEPATKRQSREYEDGPLSLVMPKKPSDSTDLASPKTMGNRASIPLKDIHVNGQLPAAEEISGKATANSLVSSEWGNPSRNTDVEKPSKENESTKEPSLLQYLCVQSPA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 E2F transcription factor 7
别名 230073C2, E2F-7, Transcription factor E2F7
计算分子量 911 aa, 100 kDa
GenBank蛋白编号BC136366
基因名称 E2F7
Gene ID (NCBI) 144455
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ96AV8
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

E2F7 also named as E2F transcription factor 7 is a 911 amino acid protein, which belongs to the E2F/DP family. E2F7 localizes in the nucleus and mainly forms homodimers and, to a lesser extent, heterodimers with E2F8. E2F7 as a transcription factor involves in various processes such as angiogenesis, polyploidization of specialized cells and DNA damage response. E2F7 mainly forms homodimers and to a lesser extent, heterodimers with E2F8. (PMID: 18194653 )

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