验证数据展示
经过测试的应用
Positive WB detected in | TDP-43-flag Transfected HEK-293T cells |
Positive IP detected in | Transfected HEK-293 cells |
Positive IF/ICC detected in | Transfected HEK-293T cells |
Positive FC (Intra) detected in | Transfected HEK-293T cells |
推荐稀释比
应用 | 推荐稀释比 |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunofluorescence (IF)/ICC | IF/ICC : 1:125-1:500 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
80801-2-RR targets DYKDDDDK tag in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with recombinant protein samples.
经测试应用 | WB, IF/ICC, FC (Intra), IP, ELISA Application Description |
文献引用应用 | WB, IHC, IF, IP |
经测试反应性 | recombinant protein |
文献引用反应性 | mouse |
免疫原 | DYKDDDDK tag fusion protein Ag2329 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Recombinant |
产品类型 | Antibody |
全称 | Flag Tag |
别名 | 3Flag, Flag, DDDDKtag, DDDDK tag, DDDDK |
基因名称 | Flag Tag |
Gene ID (NCBI) | 8 |
RRID | AB_3670488 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein A purfication |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. The DYKDDDDK(FLAG) peptide has been used extensively as a general tag in expression vectors. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion. N-terminal DDDDK vectors provide an Ek cleavage site for removal of the fusion tag. The DDDDK peptide is likely to be located on the surface of a fusion protein because of its hydrophilic nature. As a result, the DDDDK peptide is more likely to be accessible to antibodies. A DDDDK-tag can be used in many different assays that require recognition by an antibody, such as western blotting, immunocytochemistry, immunoprecipitation, flow cytometry, protein purification, and in the study of protein-protein interactions, cell ultrastructure, and protein localization and so on. This antibody is a rabbit recombinant antibody raised against 3xFlag (3xDYKDDDDKT) sequence and recognizes the (1x) and (3x)DYKDDDDK peptide and detects DDDDK-tagged proteins. Anti-FLAG is a registered trademark of Sigma-Aldrich Biotechnology.
实验方案
Product Specific Protocols | |
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WB protocol for DYKDDDDK tag antibody 80801-2-RR | Download protocol |
IF protocol for DYKDDDDK tag antibody 80801-2-RR | Download protocol |
IP protocol for DYKDDDDK tag antibody 80801-2-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Virus Res ZFP36 Facilitates Senecavirus A (SVA) Replication by Inhibiting the Production of Type I Interferon | ||
FASEB J The SLE-associated TREX1-P212fs mutation disrupts ER association leading to type I interferonopathy |