CoraLite® Plus 647-conjugated DYKDDDDK tag Recombinant antibody (Binds to FLAG® tag epitope)

DYKDDDDK tag Uni-rAbTM Recombinant Antibody for FC (Intra)
Cat No. CL647-80801-2

产品说明书

CloneNo. 240568A11

宿主/亚型

Rabbit / IgG

种属反应性

recombinant protein

应用

FC (Intra)

3Flag, Ag2329, Flag, DDDDK, DDDDK tag

缓冲液配方:  PBS and Azide
PBS and Azide
规格: 

-/ -


经过测试的应用

Positive FC (Intra) detected inHEK-293T transfected with TDP-43-flag
For other applications, we recommend the unconjugated version of this antibody, 80801-2-PBS

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-80801-2 targets DYKDDDDK tag in FC (Intra) applications and shows reactivity with recombinant protein samples.

经测试应用 FC (Intra) Application Description
经测试反应性 recombinant protein
免疫原 DYKDDDDK tag fusion protein Ag2329 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 Flag Tag
别名 3Flag, Ag2329, Flag, DDDDK, DDDDK tag
基因名称 Flag Tag
Gene ID (NCBI) 8
偶联类型 CoraLite® Plus 647 Fluorescent Dye
最大激发/发射波长654 nm / 674 nm
形式 Liquid
纯化方式Protein A purification
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. The DYKDDDDK(FLAG) peptide has been used extensively as a general tag in expression vectors. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion. N-terminal DDDDK vectors provide an Ek cleavage site for removal of the fusion tag. The DDDDK peptide is likely to be located on the surface of a fusion protein because of its hydrophilic nature. As a result, the DDDDK peptide is more likely to be accessible to antibodies. A DDDDK-tag can be used in many different assays that require recognition by an antibody, such as western blotting, immunocytochemistry, immunoprecipitation, flow cytometry, protein purification, and in the study of protein-protein interactions, cell ultrastructure, and protein localization and so on. This antibody is a rabbit recombinant antibody raised against 3xFlag (3xDYKDDDDKT) sequence and recognizes the (1x) and (3x)DYKDDDDK peptide and detects DDDDK-tagged proteins. Anti-FLAG is a registered trademark of Sigma-Aldrich Biotechnology.

实验方案

Product Specific Protocols
FC protocol for CL Plus 647 DYKDDDDK tag antibody CL647-80801-2Download protocol
Standard Protocols
Click here to view our Standard Protocols
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