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CoraLite® Plus 488-conjugated DNAJB1 Recombinant antibody

DNAJB1 Recombinant Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

4K3

Cat No : CL488-82676

Print datasheet

Synonyms

DnaJ protein homolog 1, DNAJ1, DNAJB1, hDj 1, Hdj1, Heat shock 40 kDa protein 1, Heat shock protein 40, Hsp40, HSPF1, Human DnaJ protein 1, Sis1

验证数据展示

  • IF Staining of HeLa using CL488-82676

    IF Staining of HeLa using CL488-82676

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 DNAJB1 antibody (CL488-82676, Clone: 4K3 ) at dilution of 1:200, CL594-phalloidin (red).

  • FC experiment of HeLa using CL488-82676

    FC experiment of HeLa using CL488-82676

    1X10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human DNAJB1 (CL488-82676, Clone:4K3) (red), or 0.8 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive FC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy DNAJB1 Ready-To-Use IHC Kit. DNAJB1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 82676-1-PBS

推荐稀释比

ApplicationDilution
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-82676 targets DNAJB1 in FC (Intra) applications and shows reactivity with Human, mouse, rat samples.

Tested Applications FC (Intra)
Tested Reactivity Human, mouse, rat
Immunogen DNAJB1 fusion protein Ag3830 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name DnaJ (Hsp40) homolog, subfamily B, member 1
Synonyms DnaJ protein homolog 1, DNAJ1, DNAJB1, hDj 1, Hdj1, Heat shock 40 kDa protein 1, Heat shock protein 40, Hsp40, HSPF1, Human DnaJ protein 1, Sis1
Calculated Molecular Weight 340 aa, 40 kDa
Observed Molecular Weight 37-40 kDa
GenBank Accession NumberBC019827
Gene Symbol DNAJB1
Gene ID (NCBI) 3337
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP25685
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DNAJB1 is a member of the heat shock 40 protein family and has been involved in a variety of cellular processes, including the proteasome pathway, endoplasmic reticulum stress and viral infection. DNAJB1-PRKACA gene fusions have been considered diagnostic for fibrolamellar hepatocellular carcinoma.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 DNAJB1 antibody CL488-82676Download protocol
Standard Protocols
Click here to view our Standard Protocols