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验证数据展示

  • WB analysis using 82676-1-RR (same clone as 82676-1-PBS)

    WB analysis using 82676-1-RR (same clone as 82676-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82676-1-RR (DNAJB1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82676-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse lung using 82676-1-RR (same clone as 82676-1-PBS)

    IP experiment of mouse lung using 82676-1-RR (same clone as 82676-1-PBS)

    IP result of anti-DNAJB1 (IP:82676-1-RR, 4ug; Detection:82676-1-RR 1:5000) with mouse lung tissue lysate 1120 ug. This data was developed using the same antibody clone with 82676-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 82676-1-RR (same clone as 82676-1-PBS)

    IHC staining of human colon cancer using 82676-1-RR (same clone as 82676-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 82676-1-RR (DNAJB1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82676-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with DNAJB1 antibody included
  • IHC staining of human colon cancer using 82676-1-RR (same clone as 82676-1-PBS)

    IHC staining of human colon cancer using 82676-1-RR (same clone as 82676-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 82676-1-RR (DNAJB1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82676-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with DNAJB1 antibody included
  • IF Staining of HeLa using 82676-1-RR (same clone as 82676-1-PBS)

    IF Staining of HeLa using 82676-1-RR (same clone as 82676-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using DNAJB1 antibody (82676-1-RR, Clone: 4K3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 82676-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82676-1-RR

    Affinity and Kinetic Characterization of 82676-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82676-1-RR against Human DNAJB1 were performed. The affinity constant is 0.213 nM.

DNAJB1 Recombinant monoclonal antibody, PBS Only

DNAJB1 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 82676-1-PBS

产品说明书

CloneNo. 4K3

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

hDj-1, Hdj1, hDj 1, DNAJ1, DnaJ protein homolog 1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy DNAJB1 Ready-To-Use IHC Kit. DNAJB1 primary antibody included.

产品信息

82676-1-PBS targets DNAJB1 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag3830

Product name: Recombinant human DNAJB1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-340 aa of BC019827

Sequence: MGKDYYQTLGLARGASDEEIKRAYRRQALRYHPDKNKEPGAEEKFKEIAEAYDVLSDPRKREIFDRYGEEGLKGSGPSGGSGGGANGTSFSYTFHGDPHAMFAEFFGGRNPFDTFFGQRNGEEGMDIDDPFSGFPMGMGGFTNVNFGRSRSAQEPARKKQDPPVTHDLRVSLEEIYSGCTKKMKISHKRLNPDGKSIRNEDKILTIEVKKGWKEGTKITFPKEGDQTSNNIPADIVFVLKDKPHNIFKRDGSDVIYPARISLREALCGCTVNVPTLDGRTIPVVFKDVIRPGMRRKVPGEGLPLPKTPEKRGDLIIEFEVIFPERIPQTSRTVLEQVLPI

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 DnaJ (Hsp40) homolog, subfamily B, member 1
别名 hDj-1, Hdj1, hDj 1, DNAJ1, DnaJ protein homolog 1
计算分子量 340 aa, 40 kDa
观测分子量 37-40 kDa
GenBank蛋白编号BC019827
基因名称 DNAJB1
Gene ID (NCBI) 3337
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP25685
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

DNAJB1 is a member of the heat shock 40 protein family and has been involved in a variety of cellular processes, including the proteasome pathway, endoplasmic reticulum stress and viral infection. DNAJB1-PRKACA gene fusions have been considered diagnostic for fibrolamellar hepatocellular carcinoma.

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