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Host
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Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
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Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 67422-1-Ig (same clone as 67422-1-PBS)

    WB analysis using 67422-1-Ig (same clone as 67422-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67422-1-Ig (DNAJB1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67422-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 67422-1-Ig (same clone as 67422-1-PBS)

    FC experiment of HeLa using 67422-1-Ig (same clone as 67422-1-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human DNAJB1 (67422-1-Ig, Clone:1E8E10) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 67422-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 67422-1-Ig (same clone as 67422-1-PBS)

    IF Staining of HeLa using 67422-1-Ig (same clone as 67422-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using DNAJB1 antibody (67422-1-Ig, Clone: 1E8E10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67422-1-PBS in a different storage buffer formulation.

DNAJB1 Monoclonal antibody, PBS Only

DNAJB1 Monoclonal Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 67422-1-PBS

产品说明书

CloneNo. 1E8E10

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IF/ICC, FC (Intra), Indirect ELISA

别名

1E8E10, DnaJ protein homolog 1, DNAJ1, hDj 1, Hdj1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy DNAJB1 Ready-To-Use IHC Kit. DNAJB1 primary antibody included.

产品信息

67422-1-PBS targets DNAJB1 in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human
免疫原 DNAJB1 fusion protein Ag3830 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 DnaJ (Hsp40) homolog, subfamily B, member 1
别名 1E8E10, DnaJ protein homolog 1, DNAJ1, hDj 1, Hdj1
计算分子量 340 aa, 40 kDa
观测分子量 38 kDa
GenBank蛋白编号BC019827
基因名称 DNAJB1
Gene ID (NCBI) 3337
RRIDAB_2882662
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP25685
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

DNAJB1 is a member of the heat shock 40 protein family and has been involved in a variety of cellular processes, including the proteasome pathway, endoplasmic reticulum stress and viral infection. DNAJB1-PRKACA gene fusions have been considered diagnostic for fibrolamellar hepatocellular carcinoma.