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验证数据展示

  • WB analysis using 67422-1-Ig

    WB analysis using 67422-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67422-1-Ig (DNAJB1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • FC experiment of HeLa using 67422-1-Ig

    FC experiment of HeLa using 67422-1-Ig

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human DNAJB1 (67422-1-Ig, Clone:1E8E10) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • IF Staining of HeLa using 67422-1-Ig

    IF Staining of HeLa using 67422-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using DNAJB1 antibody (67422-1-Ig, Clone: 1E8E10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red).

DNAJB1 Monoclonal antibody

DNAJB1 Monoclonal Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 67422-1-Ig

产品说明书

CloneNo. 1E8E10
引用文献(1)

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IF/ICC, FC (Intra), ELISA

别名

1E8E10, DnaJ protein homolog 1, DNAJ1, hDj 1, Hdj1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy DNAJB1 Ready-To-Use IHC Kit. DNAJB1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

67422-1-Ig targets DNAJB1 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, FC (Intra), ELISA Application Description
文献引用应用WB
经测试反应性 human
文献引用反应性human
免疫原 DNAJB1 fusion protein Ag3830 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 DnaJ (Hsp40) homolog, subfamily B, member 1
别名 1E8E10, DnaJ protein homolog 1, DNAJ1, hDj 1, Hdj1
计算分子量 340 aa, 40 kDa
观测分子量 38 kDa
GenBank蛋白编号BC019827
基因名称 DNAJB1
Gene ID (NCBI) 3337
RRIDAB_2882662
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP25685
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DNAJB1 is a member of the heat shock 40 protein family and has been involved in a variety of cellular processes, including the proteasome pathway, endoplasmic reticulum stress and viral infection. DNAJB1-PRKACA gene fusions have been considered diagnostic for fibrolamellar hepatocellular carcinoma.

实验方案

Product Specific Protocols
WB protocol for DNAJB1 antibody 67422-1-IgDownload protocol
IF protocol for DNAJB1 antibody 67422-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Mol Cell Biol

An Important Role for RPRD1B in the Heat Shock Response

Authors - Simona Cugusi
View Article