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验证数据展示

  • FC experiment of HeLa using CL488-67422

    FC experiment of HeLa using CL488-67422

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human DNAJB1 (CL488-67422, Clone:1E8E10) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • IF Staining of HeLa using CL488-67422

    IF Staining of HeLa using CL488-67422

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 488 DNAJB1 antibody (CL488-67422, Clone: 1E8E10 ) at dilution of 1:200.

CoraLite® Plus 488-conjugated DNAJB1 Monoclonal antibody

DNAJB1 Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-67422

产品说明书

CloneNo. 1E8E10

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

IF/ICC, FC (Intra)

别名

1E8E10, DnaJ protein homolog 1, DNAJ1, hDj 1, Hdj1

缓冲液配方:  PBS, Proclin 300, BSA, Glycerol
PBS, Proclin 300, BSA, Glycerol
偶联物:  CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy DNAJB1 Ready-To-Use IHC Kit. DNAJB1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67422-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67422 targets DNAJB1 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human
免疫原

CatNo: Ag3830

Product name: Recombinant human DNAJB1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-340 aa of BC019827

Sequence: MGKDYYQTLGLARGASDEEIKRAYRRQALRYHPDKNKEPGAEEKFKEIAEAYDVLSDPRKREIFDRYGEEGLKGSGPSGGSGGGANGTSFSYTFHGDPHAMFAEFFGGRNPFDTFFGQRNGEEGMDIDDPFSGFPMGMGGFTNVNFGRSRSAQEPARKKQDPPVTHDLRVSLEEIYSGCTKKMKISHKRLNPDGKSIRNEDKILTIEVKKGWKEGTKITFPKEGDQTSNNIPADIVFVLKDKPHNIFKRDGSDVIYPARISLREALCGCTVNVPTLDGRTIPVVFKDVIRPGMRRKVPGEGLPLPKTPEKRGDLIIEFEVIFPERIPQTSRTVLEQVLPI

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 DnaJ (Hsp40) homolog, subfamily B, member 1
别名 1E8E10, DnaJ protein homolog 1, DNAJ1, hDj 1, Hdj1
计算分子量 340 aa, 40 kDa
观测分子量 38 kDa
GenBank蛋白编号BC019827
基因名称 DNAJB1
Gene ID (NCBI) 3337
RRIDAB_2923772
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式Liquid
纯化方式Protein G purification
UNIPROT IDP25685
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DNAJB1 is a member of the heat shock 40 protein family and has been involved in a variety of cellular processes, including the proteasome pathway, endoplasmic reticulum stress and viral infection. DNAJB1-PRKACA gene fusions have been considered diagnostic for fibrolamellar hepatocellular carcinoma.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 DNAJB1 antibody CL488-67422Download protocol
IF protocol for CL Plus 488 DNAJB1 antibody CL488-67422Download protocol
Standard Protocols
Click here to view our Standard Protocols
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