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验证数据展示

  • WB analysis using 67422-1-Ig

    WB analysis using 67422-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67422-1-Ig (DNAJB1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • FC experiment of HeLa using 67422-1-Ig

    FC experiment of HeLa using 67422-1-Ig

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human DNAJB1 (67422-1-Ig, Clone:1E8E10) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

DNAJB1 Monoclonal antibody

DNAJB1 Monoclonal Antibody for WB, FC (Intra), ELISA
Cat No. 67422-1-Ig

产品说明书

CloneNo. 1E8E10
引用文献(1)

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, FC (Intra), ELISA

别名

hDj-1, Hdj1, hDj 1, DNAJ1, DnaJ protein homolog 1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy DNAJB1 Ready-To-Use IHC Kit. DNAJB1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

67422-1-Ig targets DNAJB1 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), ELISA Application Description
文献引用应用WB
经测试反应性 human
文献引用反应性human
免疫原 DNAJB1 fusion protein Ag3830 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 DnaJ (Hsp40) homolog, subfamily B, member 1
别名 hDj-1, Hdj1, hDj 1, DNAJ1, DnaJ protein homolog 1
计算分子量 340 aa, 40 kDa
观测分子量 38 kDa
GenBank蛋白编号BC019827
基因名称 DNAJB1
Gene ID (NCBI) 3337
RRIDAB_2882662
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP25685
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DNAJB1 is a member of the heat shock 40 protein family and has been involved in a variety of cellular processes, including the proteasome pathway, endoplasmic reticulum stress and viral infection. DNAJB1-PRKACA gene fusions have been considered diagnostic for fibrolamellar hepatocellular carcinoma.

实验方案

Product Specific Protocols
WB protocol for DNAJB1 antibody 67422-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Mol Cell Biol

An Important Role for RPRD1B in the Heat Shock Response

Authors - Simona Cugusi
View Article