验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HEK-293 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-85220-5 targets DFFA,DFF45 in applications and shows reactivity with human, rat samples.
| 经测试应用 | IF/ICC Application Description |
| 经测试反应性 | human, rat |
| 免疫原 | DFFA,DFF45 fusion protein Ag0244 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | DNA fragmentation factor, 45kDa, alpha polypeptide |
| 别名 | DFF45, DFFA, 242915D6, DFF alpha, DFF1 |
| 计算分子量 | 37 kDa |
| 观测分子量 | 45 kDa |
| GenBank蛋白编号 | BC000037 |
| 基因名称 | DFFA |
| Gene ID (NCBI) | 1676 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | O00273 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Apoptosis is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF), heterodimer of 40-kDa (DFFB) and 45-kDa (DFFA) subunits, is one of the major endonucleases responsible for internucleosomal DNA cleavage during apoptosis. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF activated after cleavage of DFFA by caspase-3 followed by its dissociation from DFFB. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 DFFA,DFF45 antibody CL488-85220-5 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |