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验证数据展示

  • WB analysis using 85220-5-RR

    WB analysis using 85220-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85220-5-RR (DFFA,DFF45 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 85220-5-RR

    IF Staining of HeLa using 85220-5-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using DFFA,DFF45 antibody (85220-5-RR, Clone: 242915D6 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 85220-5-RR

    Affinity and Kinetic Characterization of 85220-5-RR

    Biolayer interferometry (BLl) kinetic assays of 85220-5-RR against Human DFFA,DFF45 were performed. The affinity constant is 1.47 nM.

DFFA,DFF45 Recombinant antibody

DFFA,DFF45 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 85220-5-RR

产品说明书

CloneNo. 242915D6

宿主/亚型

Rabbit / IgG

种属反应性

human, rat

应用

WB, IF/ICC, ELISA

别名

DFF45, DFFA, 242915D6, DFF alpha, DFF1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, A549 cells, HEK-293 cells, K-562 cells, MCF-7 cells, C6 cells, rat brain tissue
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85220-5-RR targets DFFA,DFF45 in WB, IF/ICC, ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, rat
免疫原 DFFA,DFF45 fusion protein Ag0244 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 DNA fragmentation factor, 45kDa, alpha polypeptide
别名 DFF45, DFFA, 242915D6, DFF alpha, DFF1
计算分子量 37 kDa
观测分子量 45 kDa
GenBank蛋白编号BC000037
基因名称 DFFA
Gene ID (NCBI) 1676
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO00273
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Apoptosis is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF), heterodimer of 40-kDa (DFFB) and 45-kDa (DFFA) subunits, is one of the major endonucleases responsible for internucleosomal DNA cleavage during apoptosis. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF activated after cleavage of DFFA by caspase-3 followed by its dissociation from DFFB. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis.

实验方案

Product Specific Protocols
WB protocol for DFFA,DFF45 antibody 85220-5-RRDownload protocol
IF protocol for DFFA,DFF45 antibody 85220-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols