• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 85220-5-RR (same clone as 85220-5-PBS)

    WB analysis using 85220-5-RR (same clone as 85220-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85220-5-RR (DFFA,DFF45 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85220-5-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 85220-5-RR (same clone as 85220-5-PBS)

    IF Staining of HeLa using 85220-5-RR (same clone as 85220-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using DFFA,DFF45 antibody (85220-5-RR, Clone: 242915D6 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85220-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 85220-5-RR

    Affinity and Kinetic Characterization of 85220-5-RR

    Biolayer interferometry (BLl) kinetic assays of 85220-5-RR against Human DFFA,DFF45 were performed. The affinity constant is 1.47 nM.

DFFA,DFF45 Recombinant antibody, PBS Only

DFFA,DFF45 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 85220-5-PBS

产品说明书

CloneNo. 242915D6

宿主/亚型

Rabbit / IgG

种属反应性

human, rat

应用

WB, IF/ICC, Indirect ELISA

别名

DFF45, DFFA, 242915D6, DFF alpha, DFF1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

产品信息

85220-5-PBS targets DFFA,DFF45 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, rat
免疫原 DFFA,DFF45 fusion protein Ag0244 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 DNA fragmentation factor, 45kDa, alpha polypeptide
别名 DFF45, DFFA, 242915D6, DFF alpha, DFF1
计算分子量 37 kDa
观测分子量 45 kDa
GenBank蛋白编号BC000037
基因名称 DFFA
Gene ID (NCBI) 1676
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO00273
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Apoptosis is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF), heterodimer of 40-kDa (DFFB) and 45-kDa (DFFA) subunits, is one of the major endonucleases responsible for internucleosomal DNA cleavage during apoptosis. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF activated after cleavage of DFFA by caspase-3 followed by its dissociation from DFFB. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis.