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验证数据展示

  • WB analysis using 82428-1-RR (same clone as 82428-1-PBS)

    WB analysis using 82428-1-RR (same clone as 82428-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82428-1-RR (Cytokeratin 20 antibody) at dilution of 1:22000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82428-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 82428-1-RR (same clone as 82428-1-PBS)

    IHC staining of human colon cancer using 82428-1-RR (same clone as 82428-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 82428-1-RR (Cytokeratin 20 antibody) at dilution of 1:6000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82428-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with KRT20 antibody included
  • IHC staining of human colon cancer using 82428-1-RR (same clone as 82428-1-PBS)

    IHC staining of human colon cancer using 82428-1-RR (same clone as 82428-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 82428-1-RR (Cytokeratin 20 antibody) at dilution of 1:6000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82428-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with KRT20 antibody included
  • IF Staining of human colon cancer using 82428-1-RR (same clone as 82428-1-PBS)

    IF Staining of human colon cancer using 82428-1-RR (same clone as 82428-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human colon cancer tissue using Cytokeratin 20 antibody (82428-1-RR, Clone: 2H10 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82428-1-PBS in a different storage buffer formulation.

  • IF Staining of human colon cancer using 82428-1-RR (same clone as 82428-1-PBS)

    IF Staining of human colon cancer using 82428-1-RR (same clone as 82428-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human colon cancer tissue using Cytokeratin 20 antibody (82428-1-RR, Clone: 2H10 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82428-1-PBS in a different storage buffer formulation.

  • IF Staining of HT-29 using 82428-1-RR (same clone as 82428-1-PBS)

    IF Staining of HT-29 using 82428-1-RR (same clone as 82428-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HT-29 cells using Cytokeratin 20 antibody (82428-1-RR, Clone: 2H10 ) at dilution of 1:600 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 82428-1-PBS in a different storage buffer formulation.

  • FC experiment of HT-29 using 82428-1-RR (same clone as 82428-1-PBS)

    FC experiment of HT-29 using 82428-1-RR (same clone as 82428-1-PBS)

    1X10^6 HT-29 cells were intracellularly stained with 0.4 ug Anti-Human Cytokeratin 20 (82428-1-RR, Clone:2H10) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 82428-1-PBS in a different storage buffer formulation.

Cytokeratin 20 Recombinant monoclonal antibody, PBS Only

Cytokeratin 20 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA
Cat No. 82428-1-PBS

产品说明书

CloneNo. 2H10

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat, pig

应用

WB, IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA

别名

KRT20, CK 20, CK20, CK-20, Cytokeratin-20

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy KRT20 Ready-To-Use IHC Kit. KRT20 primary antibody included.

产品信息

82428-1-PBS targets Cytokeratin 20 in WB, IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, mouse, rat, pig
免疫原

CatNo: Ag11308

Product name: Recombinant human KRT20 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 74-424 aa of BC031559

Sequence: MQNLNDRLASYLEKVRTLEQSNSKLEVQIKQWYETNAPRAGRDYSAYYRQIEELRSQIKDAQLQNARCVLQIDNAKLAAEDFRLKYETERGIRLTVEADLQGLNKVFDDLTLHKTDLEIQIEELNKDLALLKKEHQEEVDGLHKHLGNTVNVEVDAAPGLNLGVIMNEMRQKYEVMAQKNLQEAKEQFERQTAVLQQQVTVNTEELKGTEVQLTELRRTSQSLEIELQSHLSMKESLEHTLEETKARYSSQLANLQSLLSSLEAQLMQIRSNMERQNNEYHILLDIKTRLEQEIATYRRLLEGEDVKTTEYQLSTLEERDIKKTRKIKTVVQEVVDGKVVSSEVKEVEENI

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 keratin 20
别名 KRT20, CK 20, CK20, CK-20, Cytokeratin-20
计算分子量 424 aa, 48 kDa
观测分子量46 kDa
GenBank蛋白编号BC031559
基因名称 Cytokeratin 20
Gene ID (NCBI) 54474
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP35900
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin 20 is a type I cytokeratin. It is a major cellular protein of mature enterocytes and goblet cells and is specifically found in the gastric and intestinal mucosa.

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