• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

Cytokeratin 20 Recombinant antibody

Cytokeratin 20 Recombinant Antibody for FC, IF/ICC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat, pig

Applications

FC, IF/ICC, IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

2H10

Cat No : 82428-1-RR

Print datasheet

Synonyms

CK 20, CK20, Cytokeratin 20, K20, keratin 20, KRT20, KRT21, Protein IT

验证数据展示

  • WB analysis using 82428-1-RR

    WB analysis using 82428-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82428-1-RR (Cytokeratin 20 antibody) at dilution of 1:22000 incubated at room temperature for 1.5 hours.

  • IHC staining of human appendicitis using 82428-1-RR

    IHC staining of human appendicitis using 82428-1-RR

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 82428-1-RR (Cytokeratin 20 antibody) at dilution of 1:1100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with KRT20 antibody included
  • IHC staining of human appendicitis using 82428-1-RR

    IHC staining of human appendicitis using 82428-1-RR

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 82428-1-RR (Cytokeratin 20 antibody) at dilution of 1:1100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with KRT20 antibody included
  • IF Staining of HT-29 using 82428-1-RR

    IF Staining of HT-29 using 82428-1-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HT-29 cells using Cytokeratin 20 antibody (82428-1-RR, Clone: 2H10 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • FC experiment of HT-29 using 82428-1-RR

    FC experiment of HT-29 using 82428-1-RR

    1X10^6 HT-29 cells were intracellularly stained with 0.4 ug Anti-Human Cytokeratin 20 (82428-1-RR, Clone:2H10) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inHT-29 cells, COLO 320 cells, pig colon tissue, mouse colon tissue, rat colon tissue
Positive IHC detected inhuman appendicitis tissue, human colon cancer tissue, human urothelial carcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHT-29 cells
Positive FC detected inHT-29 cells
Planning an IHC experiment? We recommend our IHCeasy KRT20 Ready-To-Use IHC Kit. KRT20 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:750-1:3000
Immunofluorescence (IF)IF : 1:500-1:2000
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82428-1-RR targets Cytokeratin 20 in WB, IF, FC, IHC, ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

Tested Applications FC, IF/ICC, IHC, WB, ELISA
Tested Reactivity Human, mouse, rat, pig
Immunogen Cytokeratin 20 fusion protein Ag11308 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name keratin 20
Synonyms CK 20, CK20, Cytokeratin 20, K20, keratin 20, KRT20, KRT21, Protein IT
Calculated Molecular Weight 424 aa, 48 kDa
Observed Molecular Weight46 kDa
GenBank Accession NumberBC031559
Gene Symbol KRT20
Gene ID (NCBI) 54474
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP35900
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin 20 is a type I cytokeratin. It is a major cellular protein of mature enterocytes and goblet cells and is specifically found in the gastric and intestinal mucosa.

实验方案

Product Specific Protocols
WB protocol for Cytokeratin 20 antibody 82428-1-RRDownload protocol
IHC protocol for Cytokeratin 20 antibody 82428-1-RRDownload protocol
IF protocol for Cytokeratin 20 antibody 82428-1-RRDownload protocol
FC protocol for Cytokeratin 20 antibody 82428-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols