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验证数据展示

  • WB analysis using 82428-1-RR

    WB analysis using 82428-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82428-1-RR (Cytokeratin 20 antibody) at dilution of 1:22000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 82428-1-RR

    IHC staining of human colon cancer using 82428-1-RR

    Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 82428-1-RR (Cytokeratin 20 antibody) at dilution of 1:6000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with KRT20 antibody included
  • IF Staining of human colon cancer using 82428-1-RR

    IF Staining of human colon cancer using 82428-1-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human colon cancer tissue using Cytokeratin 20 antibody (82428-1-RR, Clone: 2H10 ) at dilution of 1:500 and CoraLite®647-conjugated F(ab')2 Fragment Goat Anti-Rabbit IgG (H+L) (SA00014-9), CoraLite®594 CD68 antibody (CL594-25747, red), CoraLite® Plus 488 Collagen Type I antibody (CL488-67288, Clone: 1E9A7, green). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon cancer using 82428-1-RR

    IHC staining of human colon cancer using 82428-1-RR

    Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 82428-1-RR (Cytokeratin 20 antibody) at dilution of 1:6000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with KRT20 antibody included
  • IF Staining of human colon cancer using 82428-1-RR

    IF Staining of human colon cancer using 82428-1-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human colon cancer tissue using Cytokeratin 20 antibody (82428-1-RR, Clone: 2H10 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of human colon cancer using 82428-1-RR

    IF Staining of human colon cancer using 82428-1-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human colon cancer tissue using Cytokeratin 20 antibody (82428-1-RR, Clone: 2H10 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colorectal cancer using 82428-1-RR

    IHC staining of human colorectal cancer using 82428-1-RR

    Immunohistochemical analysis of paraffin-embedded human colorectal cancer tissue slide using 82428-1-RR (Cytokeratin 20 antibody) at dilution of 1:6000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.

  • IHC staining of human colorectal cancer using 82428-1-RR

    IHC staining of human colorectal cancer using 82428-1-RR

    Immunohistochemical analysis of paraffin-embedded human colorectal cancer tissue slide using 82428-1-RR (Cytokeratin 20 antibody) at dilution of 1:6000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.

  • IHC staining of human appendicitis using 82428-1-RR

    IHC staining of human appendicitis using 82428-1-RR

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 82428-1-RR (Cytokeratin 20 antibody) at dilution of 1:6000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.

  • IHC staining of human appendicitis using 82428-1-RR

    IHC staining of human appendicitis using 82428-1-RR

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 82428-1-RR (Cytokeratin 20 antibody) at dilution of 1:6000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with KRT20 antibody included
  • IHC staining of human colorectal cancer using 82428-1-RR

    IHC staining of human colorectal cancer using 82428-1-RR

    Immunohistochemical analysis of paraffin-embedded human colorectal cancer tissue slide using 82428-1-RR (Cytokeratin 20 antibody) at dilution of 1:6000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with KRT20 antibody included
  • IHC staining of human colorectal cancer using 82428-1-RR

    IHC staining of human colorectal cancer using 82428-1-RR

    Immunohistochemical analysis of paraffin-embedded human colorectal cancer tissue slide using 82428-1-RR (Cytokeratin 20 antibody) at dilution of 1:6000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with KRT20 antibody included
  • IF Staining of HT-29 using 82428-1-RR

    IF Staining of HT-29 using 82428-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HT-29 cells using Cytokeratin 20 antibody (82428-1-RR, Clone: 2H10 ) at dilution of 1:600 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • FC experiment of HT-29 using 82428-1-RR

    FC experiment of HT-29 using 82428-1-RR

    1X10^6 HT-29 cells were intracellularly stained with 0.4 ug Anti-Human Cytokeratin 20 (82428-1-RR, Clone:2H10) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

Cytokeratin 20 Recombinant monoclonal antibody

Cytokeratin 20 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA
Cat No. 82428-1-RR

产品说明书

CloneNo. 2H10
引用文献(4)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat, pig

应用

WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA and More (1)

别名

KRT20, CK 20, CK20, CK-20, Cytokeratin-20

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive WB detected inHT-29 cells, COLO 320 cells, pig colon tissue, mouse colon tissue, rat colon tissue
Positive IHC detected inhuman colon cancer tissue, human colon tissue, human colorectal cancer tissue, human urothelial carcinoma tissue, human appendicitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman colon cancer tissue, human colon tissue
Positive IF/ICC detected inHT-29 cells
Positive FC (Intra) detected inHT-29 cells
Planning an IHC experiment? We recommend our IHCeasy KRT20 Ready-To-Use IHC Kit. KRT20 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:3000-1:12000
Immunofluorescence (IF)-PIF-P : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:300-1:1200
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82428-1-RR targets Cytokeratin 20 in WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA Application Description
文献引用应用IHC, IF
经测试反应性 human, mouse, rat, pig
文献引用反应性human
免疫原

CatNo: Ag11308

Product name: Recombinant human KRT20 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 74-424 aa of BC031559

Sequence: MQNLNDRLASYLEKVRTLEQSNSKLEVQIKQWYETNAPRAGRDYSAYYRQIEELRSQIKDAQLQNARCVLQIDNAKLAAEDFRLKYETERGIRLTVEADLQGLNKVFDDLTLHKTDLEIQIEELNKDLALLKKEHQEEVDGLHKHLGNTVNVEVDAAPGLNLGVIMNEMRQKYEVMAQKNLQEAKEQFERQTAVLQQQVTVNTEELKGTEVQLTELRRTSQSLEIELQSHLSMKESLEHTLEETKARYSSQLANLQSLLSSLEAQLMQIRSNMERQNNEYHILLDIKTRLEQEIATYRRLLEGEDVKTTEYQLSTLEERDIKKTRKIKTVVQEVVDGKVVSSEVKEVEENI

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 keratin 20
别名 KRT20, CK 20, CK20, CK-20, Cytokeratin-20
计算分子量 424 aa, 48 kDa
观测分子量46 kDa
GenBank蛋白编号BC031559
基因名称 Cytokeratin 20
Gene ID (NCBI) 54474
RRIDAB_3086478
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP35900
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin 20 is a type I cytokeratin. It is a major cellular protein of mature enterocytes and goblet cells and is specifically found in the gastric and intestinal mucosa.

实验方案

Product Specific Protocols
FC protocol for Cytokeratin 20 antibody 82428-1-RRDownload protocol
IF protocol for Cytokeratin 20 antibody 82428-1-RRDownload protocol
IHC protocol for Cytokeratin 20 antibody 82428-1-RRDownload protocol
WB protocol for Cytokeratin 20 antibody 82428-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Cell Death Discov

Colorectal cancer-derived osteopontin rewires macrophages into a pro-metastatic M2 state via the PI3K/AKT/CSF1-CSF1R axis.

Authors - Xiaoxia Liang
View Article
humanIF

Oncogenesis

Ubiquitin-specific protease 11 suppresses cuproptosis in colorectal cancer by regulating the ubiquitination and stability of ISCU.

Authors - Hailin Ke
View Article
humanIHC

Exp Ther Med

Appendiceal mucinous tumour resulting in autoamputation of the appendix: A case report and literature review.

Authors - Chenao Wang
View Article

bioRxiv

An immunocompetent model of MCPyV-driven Merkel cell carcinoma reveals tumor evolution under immune selection.

Authors - James M Regan
View Article
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