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验证数据展示

  • IF Staining of HeLa using CL594-60228

    IF Staining of HeLa using CL594-60228

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite®594 Cytoglobin antibody (CL594-60228, Clone: 4C7E11 ) at dilution of 1:200, CL488-Phalloidin (green).

  • IF Staining of HeLa using CL594-60228

    IF Staining of HeLa using CL594-60228

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite®594 Cytoglobin antibody (CL594-60228, Clone: 4C7E11 ) at dilution of 1:200.

CoraLite®594-conjugated Cytoglobin Monoclonal antibody

Cytoglobin Monoclonal Antibody for IF/ICC
Cat No. CL594-60228

产品说明书

CloneNo. 4C7E11

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig

应用

IF/ICC

别名

CYGB, 4C7E11, EC:1.11.1.-, EC:1.14.12.-, EC:1.15.1.1

缓冲液配方:  PBS, Proclin 300, BSA, Glycerol
PBS, Proclin 300, BSA, Glycerol
偶联物:  CoraLite®594
规格价格库存

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经过测试的应用

Positive IF/ICC detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 60228-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-60228 targets Cytoglobin in IF/ICC applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 IF/ICC Application Description
经测试反应性 human, mouse, rat, pig
免疫原

CatNo: Ag4161

Product name: Recombinant human CYGB protein

Source: e coli.-derived, T-HIS

Tag: 6*His

Domain: 1-190 aa of BC029798

Sequence: MEKVPGEMEIERRERSEELSEAERKAVQAMWARLYASCEDVGVAILVRFFVNFPSAKQYFSQFKHMEDPLEMERSPQLRKHACRVMGALNTVVENLHDPDKVSSVLALVGKAHALKHKVEPVYFKILSGVILEVVAEEFASDFPPETQRAWAKLRGLIYSHVTAAYKEVGWVQQVPNATTPPATLPSSGP

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 cytoglobin
别名 CYGB, 4C7E11, EC:1.11.1.-, EC:1.14.12.-, EC:1.15.1.1
计算分子量 190 aa, 21 kDa
观测分子量 27-29 kDa
GenBank蛋白编号BC029798
基因名称 Cytoglobin
Gene ID (NCBI) 114757
RRIDAB_2883446
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ8WWM9
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cytoglobin is a ubiquitously expressed hexacoordinate hemoglobin that may facilitate diffusion of oxygen through tissues. The protein, with calculated MW of 21 kDa, runs as a 29kd one in canine retina, kidney, liver, lung, and heart. It may be caused by posttranslational modification of the protein. Various immuno-staining patterns were reported including nuclear, cytoplasm and extracellular location.

实验方案

Product Specific Protocols
IF protocol for CL594 Cytoglobin antibody CL594-60228Download protocol
Standard Protocols
Click here to view our Standard Protocols
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