验证数据展示
经过测试的应用
| Positive WB detected in | Human heart, pig heart tissue, human heart tissue, Transfected HEK-293 cells, rabbit heart tissue |
| Positive IHC detected in | human liver tissue, human heart tissue, human bladder tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
60228-1-Ig targets Cytoglobin in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IF, IP |
| 经测试反应性 | human, mouse, rat, pig |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag4161 Product name: Recombinant human CYGB protein Source: e coli.-derived, T-HIS Tag: 6*His Domain: 1-190 aa of BC029798 Sequence: MEKVPGEMEIERRERSEELSEAERKAVQAMWARLYASCEDVGVAILVRFFVNFPSAKQYFSQFKHMEDPLEMERSPQLRKHACRVMGALNTVVENLHDPDKVSSVLALVGKAHALKHKVEPVYFKILSGVILEVVAEEFASDFPPETQRAWAKLRGLIYSHVTAAYKEVGWVQQVPNATTPPATLPSSGP 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | cytoglobin |
| 别名 | CYGB, 4C7E11, EC:1.11.1.-, EC:1.14.12.-, EC:1.15.1.1 |
| 计算分子量 | 190 aa, 21 kDa |
| 观测分子量 | 27-29 kDa |
| GenBank蛋白编号 | BC029798 |
| 基因名称 | Cytoglobin |
| Gene ID (NCBI) | 114757 |
| RRID | AB_11182383 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | Q8WWM9 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Cytoglobin is a ubiquitously expressed hexacoordinate hemoglobin that may facilitate diffusion of oxygen through tissues. The protein, with calculated MW of 21 kDa, runs as a 29 kDa one in canine retina, kidney, liver, lung, and heart. It may be caused by posttranslational modification of the protein. Various immuno-staining patterns were reported including nuclear, cytoplasm and extracellular location.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for Cytoglobin antibody 60228-1-Ig | Download protocol |
| IHC protocol for Cytoglobin antibody 60228-1-Ig | Download protocol |
| WB protocol for Cytoglobin antibody 60228-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Cell Mol Med Cytoglobin promotes sensitivity to ferroptosis by regulating p53-YAP1 axis in colon cancer cells. | ||
Mol Cell Biochem Cytoglobin augments ferroptosis through autophagic degradation of ferritin in colorectal cancer cells | ||
Cell Death Dis ΔNp63α promotes Bortezomib resistance via the CYGB-ROS axis in head and neck squamous cell carcinoma. | ||
Mol Med Rep Cytoglobin attenuates melanoma malignancy but protects melanoma cells from ferroptosis | ||
Neural Regen Res Treadmill exercise in combination with acousto-optic and olfactory stimulation improves cognitive function in APP/PS1 mice through the brain-derived neurotrophic factor- and Cygb-associated signaling pathways |
